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Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells
submitted by bonnieeichler 1 hour ago
Controlling the balance of endothelial cells (ECs) and smooth muscle cells (SMCs) in blood vessels is critically important to minimize the risk associated with vascular implants. Extracellular matrix (ECM) plays a key role in controlling the cellular balance, suggesting a promising source of cell...
 
1 dig(s) Bury Topic: Biotechnology


The use of high-solids loadings in biomass pretreatment—a review
submitted by natzobaatplonts 1 hour ago
The use of high-solids loadings (≥15% solids, w/w) in the unit operations of lignocellulose conversion processes potentially offers many advantages over lower-solids loadings, including increased sugar and ethanol concentrations and decreased production and capital costs. Since the term lignoce...
 
1 dig(s) Bury Topic: Biotechnology
Determination and control of low-level amino acid misincorporation in human thioredoxin protein produced in a recombinant Escherichia coli production system
submitted by j5onjin 1 hour ago
Escherichia coli is used extensively in the production of proteins within biotechnology for a number of therapeutic applications. Here, we discuss the production and overexpression of the potential biopharmaceutical human thioredoxin protein (rhTRX) within E. coli. Overexpression of foreign molec...
 
1 dig(s) Bury Topic: Biotechnology
A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system
submitted by yossen 1 hour ago
A new electrochemical assay for the detection of secreted alkaline phosphatase (SEAP) from transfectant HeLa cells is proposed using a microarray device and scanning electrochemical microscopy (SECM). The assay consists of two steps: the first is the incubation of a transfected cell in a microarr...
 
1 dig(s) Bury Topic: Biotechnology


Polyphenolic disaccharides endow proteins with unusual resistance to aggregation
submitted by DalDenn 1 hour ago
Protein aggregation is a common problem during the purification and formulation of therapeutic proteins. Here we report that polyphenolic disaccharides are unusually effective at preventing protein aggregation. We find that two polyphenolic glycosides – naringin and rutin – endow diverse prot...
 
1 dig(s) Bury Topic: Biotechnology
Modification of macroporous titanium tracheal implants with biodegradable structures: Tracking in vivo integration for determination of optimal in situ epithelialization conditions
submitted by blaigriinarongi 1 hour ago
Previously, we showed that macroporous titanium implants, colonized in vivo together with an epithelial graft, are viable options for tracheal replacement in sheep. To decrease the number of operating steps, biomaterial based replacements for epithelial graft and intramuscular implantation were d...
 
1 dig(s) Bury Topic: Biotechnology
High gas pressure: An innovative method for the inactivation of dried bacterial spores
submitted by AdaTyrese 1 hour ago
In this paper, an original nonthermal process to inactivate dehydrated bacterial spores is described. The use of gases such as nitrogen or argon as transmission media under high isostatic pressure led to an inactivation of over 2 logs CFU/g of Bacillus subtilis spores at 430 MPa, room temperatu...
 
1 dig(s) Bury Topic: Biotechnology
Utilisation and evaluation of CHO-specific sequence databases for mass spectrometry based proteomics
submitted by topeto 1 hour ago
Recently released sequence information on Chinese Hamster Ovary (CHO) cells promises to not only facilitate our understanding of these industrially important cell factories through direct analysis of the sequence, but also to enhance existing methodologies and allow new tools to be developed. In ...
 
1 dig(s) Bury Topic: Biotechnology
Radiotolerance of phosphatases of a Serratia sp.: Potential for the use of this organism in the biomineralization of wastes containing radionuclides
submitted by ycgrinmymoin 1 hour ago
Aqueous wastes from nuclear fuel reprocessing present special problems of radiotoxicity of the active species. Cells of Serratia sp. were found previously to accumulate high levels of hydrogen uranyl phosphate (HUP) via the activity of a phosphatase enzyme. Uranium is of relatively low radiotoxic...
 
1 dig(s) Bury Topic: Biotechnology
Thermo-responsive non-woven scaffolds for “smart” 3D cell culture
submitted by pporminteir 1 hour ago
The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(eth...
 
1 dig(s) Bury Topic: Biotechnology
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