Organics

Enantioselective Fluorination of β-Ketoamides Catalyzed by Ar-BINMOL-derived SalanCopper Complex
A facile and powerful enantioselective construction of CF containing molecules was successfully developed through asymmetric fluorination of β-ketoamides catalyzed by Ar-BINMOL-derived salan–CuII system (Ar-BINMOL=1,1′-Binaphthalene-2-α-arylmethanol-2′-ol, Cu = copper). The present catalytic system exhibited excellent enantioselectivity and a broad substrate scope for indanone-derived ...


Scale-Up Investigation of the Continuous Phase-Transfer-Catalyzed Hypochlorite Oxidation of Alcohols and Aldehydes
The use of bleach to oxidize alcohols with the aid of a phase-transfer catalyst (PTC) offers several benefits over traditional oxidants: low material cost, mild reaction conditions, and no metallic waste. Mass transport limitations often dictate overall reaction rates of such PTC reactions, and continuous-flow reactors with superior mass and heat transport performance are consequently used to e...
Straddling the tholeiitic/calc-alkaline transition: the effects of modest amounts of water on magmatic differentiation at Newberry Volcano, Oregon
Melting experiments have been performed at 1 bar (anhydrous) and 1- and 2-kbar H2O-saturated conditions to study the effect of water on the differentiation of a basaltic andesite. The starting material was a mafic pumice from the compositionally zoned tuff deposited during the ~75 ka caldera-forming eruption of Newberry Volcano, a rear-arc volcanic center in the central Oregon Cascades. Pumic...


Pre- and syn-eruptive degassing and crystallisation processes of the 2010 and 2006 eruptions of Merapi volcano, Indonesia
The 2010 eruption of Merapi (VEI 4) was the volcano’s largest since 1872. In contrast to the prolonged and effusive dome-forming eruptions typical of Merapi’s recent activity, the 2010 eruption began explosively, before a new dome was rapidly emplaced. This new dome was subsequently destroyed by explosions, generating pyroclastic density currents (PDCs), predominantly consisting of dark col...
Ylide stabilized carbenes: a computational study
High-level Density Functional Theory calculations, coupled with appropriate isodesmic reaction, are employed to investigate the effects of α-carbon, ammonium, phosphorus, and sulfur ylides, cyclization, and unsaturation on the stability, multiplicity, and reactivity of novel singlet (S) and triplet (T) carbenes. Among them the highly π-donating α-ammonium ylide is found to exert the highest ...


Cell-Based Protein Stabilization Assays for the Detection of Interactions between Small-Molecule Inhibitors and BRD4
Bromodomain protein 4 (BRD4), a member of the bromodomain and extra-terminal (BET) protein family, acts as a central element in transcriptional elongation and plays essential roles in cell proliferation. Inhibition of BRD4 binding to acetylated histone tails via its two bromodomains, BD1 and BD2, with small-molecule inhibitors has been shown to be a valid strategy to prevent cancer growth. We h...
Pinocembrin Protects the Brain against Ischemia-Reperfusion Injury and Reverses the Autophagy Dysfunction in the Penumbra Area
The aim of this study was to investigate the effects of pinocembrin on brain ischemia/reperfusion (I/R) injury and the potential involvement of autophagy activity changes in the penumbra area in the mechanisms of pinocembrin activity. Focal cerebral I/R model was induced by middle cerebral artery occlusion (MCAO) for 2 h followed by 24 h reperfusion. Pinocembrin was administered intravenously a...

Arbutus unedo L.: Chemical and Biological Properties
Arbutus unedo L. (strawberry tree) has a circum-Mediterranean distribution, being found in western, central and southern Europe, north-eastern Africa (excluding Egypt and Libya) and the Canary Islands and western Asia. Fruits of the strawberry tree are generally used for preparing alcoholic drinks (wines, liqueurs and brandies), jams, jellies and marmalades, and less frequently eaten as fresh f...
A Starting Point for Fluorescence-Based Single-Molecule Measurements in Biomolecular Research
Single-molecule fluorescence techniques are ideally suited to provide information about the structure-function-dynamics relationship of a biomolecule as static and dynamic heterogeneity can be easily detected. However, what type of single-molecule fluorescence technique is suited for which kind of biological question and what are the obstacles on the way to a successful single-molecule microsco...

New Derivatives of 3,4-Dihydroisoquinoline-3-carboxylic Acid with Free-Radical Scavenging, D-Amino Acid Oxidase, Acetylcholinesterase and Butyrylcholinesterase Inhibitory Activity
A series of 3,4-dihydroisoquinoline-3-carboxylic acid derivatives were synthesised and tested for their free-radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS·+), superoxide anion radical (O2·−) and nitric oxide radical (·NO) assays. We also studied d-amino acid oxidase (DAAO), acetylcholin...
Photochemical Aryl Radical Cyclizations to Give (E)-3-Ylideneoxindoles
(E)-3-Ylideneoxindoles are prepared in methanol in reasonable to good yields, as adducts of photochemical 5-exo-trig of aryl radicals, in contrast to previously reported analogous radical cyclizations initiated by tris(trimethylsilyl)silane and azo-initiators that gave reduced oxindole adducts.

Leveraging the complementary nature of RNA-Seq and shotgun proteomics data
RNA sequencing (RNA-Seq) and mass spectrometry-based shotgun proteomics are powerful high-throughput technologies for identifying and quantifying RNA transcripts and proteins respectively. With the increasing affordability of these technologies, many projects have started to apply both to the same samples to achieve a more comprehensive understanding of biological systems. A major analytical ch...
Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel
The sensitivity of Western blotting analysis after Phos-tag SDS-PAGE is occasionally inferior to that after normal (Phos-tag-free) SDS-PAGE under similar experimental conditions, possibly as a result of inefficient electrotransfer from the Phos-tag gel to the blotting membrane. We therefore present tips on improving the efficiency of electrotransfer of proteins in semi-dry and wet-tank blotting...
Array-based proteomic approaches to study signal transduction pathways: Prospects, merits and challenges
Very often dysfunctional aspects of various signalling networks are found to be associated with human diseases and disorders. The major characteristics of signal-transduction pathways are specificity, amplification of the signal, desensitization and integration, which is accomplished not solely, but majorly by proteins. Array-based profiling of protein-protein and other biomolecular interaction...
Proteomic analysis in Giardia duodenalis yields insights into strain virulence and antigenic variation
Giardia duodenalis is a protozoan parasite of the small intestine in vertebrates, including humans. Assemblage A of G. duodenalis is one of two discrete subtypes that infects humans, and is considered a zoonotic assemblage. Two G. duodenalis Assemblage A strains BRIS/95/HEPU/2041 and BRIS/83/HEPU/106, constituting virulent and control strains respectively, were analysed in one of the first comp...