Organics

Fatty Acid Profiles of Virgin Olive Oils from the Five Olive-Growing Zones of Extremadura (Spain)
In this study, 278 samples of virgin olive oil from 40 mills belonging to five olive-growing zones of Extremadura were evaluated according to their fatty acid composition. An analysis of variance of the fatty acid contents, and their principal group subtotals and ratios revealed significant differences at the 95 % confidence level between zones. Means were compared using Tukey’s HSD test (p...


Iron inhibits Escherichia coli topoisomerase I activity by targeting the first two zinc-binding sites in the C-terminal domain
Escherichia coli DNA topoisomerase I (TopA) contains a 67 kD N-terminal catalytic domain and a 30 kD C-terminal zinc-binding region (ZD domain) which has three adjacent tetra-cysteine zinc-binding motifs. Previous studies have shown that E. coli TopA can bind both iron and zinc, and that iron binding in TopA results in failure to unwind the negatively supercoiled DNA. Here, we report that each ...
High Resolution Crystal Structures of the Photoreceptor Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) with Three and Four-bound NAD Molecules
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the oxidative phosphorylation of D-glyceraldehyde 3-phosphate (G3P) into 1,3-diphosphoglycerate (BGP) in the presence of the NAD cofactor. GAPDH is an important drug target because of its central role in glycolysis, and non-glycolytic processes such as nuclear RNA transport, DNA replication/repair, membrane fusion and cellular apoptosis...


Slow-released NPK fertilizer encapsulated by NaAlg-g-poly(AA-co-AAm)/MMT superabsorbent nanocomposite
A novel slow released NPK fertilizer encapsulated by superabsorbent nanocomposite was prepared via in-situ free radical polymerization of sodium alginate, acrylic acid, acrylamide, and montmorillonite in the presence of fertilizer compounds. Evidence of grafting and component interactions, superabsorbent nanocomposite structure and morphology was obtained by a FT-IR, XRD and SEM techniques. The...
UV-stable paper coated with APTES-modified P25 TiO2 nanoparticles
In order to inhibit the photocatalytic degradation of organic material supports induced by small titania (TiO2) nanoparticles, highly photocatalytically active, commercially available P25-TiO2 nanoparticles were first modified with a thin layer of (3-aminopropyl)triethoxysilane (APTES), which were then deposited and fixed onto the surface of paper samples via a simple, dip-coating process in wa...


Evaluation of the ability of xanthan gum/gellan gum/hyaluronan hydrogel membranes to prevent the adhesion of postrepaired tendons
After tendon-repair surgery, adhesion between the surgical tendon and the synovial sheath is often presented resulting in poor functional repair of the tendon. This may be prevented using a commercially available mechanical barrier implant, Seprafilm, which is composed of hyaluronan (HA) and carboxymethyl cellulose hydrogels. In a rat model, prepared membranes of various compositions of gellan ...
Toughening mechanisms in interfacially modified HDPE/thermoplastic starch blends
The mechanical behavior of polymer blends containing 80wt% of HDPE and 20wt% of TPS and compatibilized with HDPE-g-MA grafted copolymer was investigated. Unmodified HDPE/TPS blends exhibit high fracture resistance, however, the interfacial modification of those blends by addition of HDPE-g-MA leads to a dramatic drop in fracture resistance. The compatibilization of HDPE/TPS blends increases the...

Three-dimensional electrospun polycaprolactone (PCL)/alginate hybrid composite scaffolds
Micro/nanofibrous scaffolds have been used widely in biomedical applications because the micro/nano-scale fibres resemble natural extracellular matrix and the high surface-to-volume ratio encourages cellular activities (attachment and proliferation). However, poor mechanical properties, low controllability of various shapes and difficulties in obtaining controllable pore structure have been obs...
Changes of structure and property of alkali soluble hydroxyethyl celluloses (HECs) and their regenerated films with the molar substitution
Hydroxyethyl celluloses (HECs) with low molar substitutions (MS) were prepared by reaction of alkali cellulose with ethylene oxide (EO) in a heterogeneous process. NMR, FTIR, WAXD, TG, solubility and tensile tests were adopted to investigate the changes of structures and properties of HECs with their MS values. NMR results showed that the hydroxyethyl groups were introduced into the cellulose c...

Carboxymethylation of an exopolysaccharide from Lachnum and effect of its derivatives on experimental chronic renal failure
Carboxymethylated polysaccharide CLEP-1b was prepared from a single component (LEP-1b) of Lachnum YM281 exopolysaccharides by molecular modification with a degree of substitution (DS) of 0.286. Infrared result proved that the carboxymethylation of LEP-1b succeeded and 13C NMR result showed that the carboxymethyl group (CH2COOH) was chemically linked to an oxygen (O) atom of the hydroxyl on C-3 ...
MDG-1, a polysaccharide from Ophiopogon japonicus, prevents high fat diet-induced obesity and increases energy expenditure in mice
Mdg-1, a water-soluble polysaccharide extracted from Ophiopogon japonicus, has potent hypoglycemic and weight control effects. We investigated the impact of MDG-1 on body weight, indirect calorimetry, body composition, plasma biochemical indices and obesity-related mitochondrial activity in diet-induced obese mice. Obese C57BL/6 mice induced by a high fat diet were given either vehicle or vehic...

Cellulose-nanofiber/polygalacturonic acid coatings with high oxygen barrier and targeted release properties
A bio-inspired coating consisting of pectin (polygalacturonic acid) and cationic cellulose nanofibers were successfully produced by the layer-by-layer method. The build-up and the morphology of the resulting coatings were studied with spectroscopic ellipsometry and atomic force microscopy, respectively. The coating was able to survive the exposure of a simulated gastric fluid, but was partially...
Protoberberine Isoquinoline Alkaloids from Arcangelisia gusanlung
HPLC-DAD-directed isolation and purification of the methanol extract of stems of Arcangelisia gusanlung H. S. Lo. led to the isolation of a new protoberberine alkaloid, gusanlung E (1), along with fourteen known derivatives 2–15, seven of which were obtained from the genus Arcangelisia for the first time. The structures and absolute stereochemistry of these compounds were elucidated on the ba...
Effect of Apitherapy Formulations against Carbon Tetrachloride-Induced Toxicity in Wistar Rats after Three Weeks of Treatment
The human body is exposed nowadays to increasing attacks by toxic compounds in polluted air, industrially processed foods, alcohol and drug consumption that increase liver toxicity, leading to more and more severe cases of hepatic disorders. The present paper aims to evaluate the influence of the apitherapy diet in Wistar rats with carbon tetrachloride-induced hepatotoxicity, by analyzing the b...
A New Cycloartane-Type Triterpenoid Saponin Xanthine Oxidase Inhibitor from Homonoia riparia Lour
A new cycloartane-type triterpenoid saponin named riparsaponin (1) was isolated from the stem of Homonoia riparia Lour together with six known compounds. The structure of riparsaponin was determined by using NMR and mass spectroscopy and X-ray crystallography techniques. Additionally, riparsaponin has a significant inhibitory effect on xanthine oxidase in vitro, and the IC50 was 11.16 nmol/mL.