Biotechnology

High-throughput screening for integrative biomaterials design: exploring advances and new trends
With the increasing need for biomaterials and tissue engineering alternatives, more accurate, rapid, and cost-saving methods and models to study biomaterial–cell interactions must be developed. We review the evolution of microarray platforms used for such studies in order to meet the criteria of complex tissue engineering biological environments. Particular aspects regarding biomaterials proc...


Complete genome sequence of the plant growth-promoting rhizobacterium Pseudomonas aurantiaca strain JD37
Pseudomonas aurantiaca Strain JD37, a Gram-negative bacterium isolated from potato rhizosphere soil (Shanghai, China), is a plant growth-promoting rhizobacterium. The JD37 genome consists of only one chromosome with no plasmids. Its genome contains genes involved plant growth promoting, biological control, and other function. Here, we present the complete genome sequence of P. aurantiaca JD37. ...
Enrichment of Plasticicumulans acidivorans at pilot-scale for PHA production on industrial wastewater
A PHA producing microbial culture dominated by P. acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, enrichment of a PHA-producing microbial community and accumulation for maximization of the cellular PHA content. after anaerobic fermentation the wastewater contained mainly VFAs (0.64±0.15 ...


Enhanced production of n-alkanes in Escherichia coli by spatial organization of biosynthetic pathway enzymes
Alkanes chemically mimic hydrocarbons found in petroleum, and their demand as biofuels is steadily increasing. Biologically, n-alkanes are produced from fatty acyl-ACPs by acyl-ACP reductases (AARs) and aldehyde deformylating oxygenases (ADOs). One of the major impediments in n-alkane biosynthesis is the low catalytic turnover rates of ADOs. Here, we studied n-alkane biosynthesis in Escherichia...
Lipid production from corn stover by the oleaginous yeast Cryptococcus curvatus
Microbial lipids produced from lignocellulosic biomass hold great promise for the biodiesel industry. These lipids usually consist of three major processes: pretreatment, enzymatic hydrolysis and lipid production. However, the conventional strategy of using biomass hydrolysates as the feedstock for lipid production suffers from low lipid coefficient and prohibitively high costs. More cost-effec...


A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
Isolation of cells harboring exogenous DNA is typically achieved by the introduction of plasmids, but its efficiency remains still low. In this study, we developed a novel strategy to obtain stable transfectants efficiently. Porcine embryonic fibroblasts were transfected with two plasmids: 1) pTransIEnd, which comprises the ubiquitous promoter, the piggyBac (PB) transposase gene, an internal ri...
Green genes: bioinformatics and systems-biology innovations drive algal biotechnology
Many species of microalgae produce hydrocarbons, polysaccharides, and other valuable products in significant amounts. However, large-scale production of algal products is not yet competitive against non-renewable alternatives from fossil fuel. Metabolic engineering approaches will help to improve productivity, but the exact metabolic pathways and the identities of the majority of the genes invo...

Metabolic variability in bioprocessing: implications of microbial phenotypic heterogeneity
Phenotypic heterogeneity is a major issue in the context of industrial bioprocessing. Stochasticity of gene expression is usually considered to be the main source of heterogeneity among microbial population, but recent evidence demonstrates that metabolic reactions can also be subject to stochasticity without any intervention of gene expression. Although metabolic heterogeneity can be encounter...
Synthetic scaffold based on a cohesin-dockerin interaction for improved production of 2,3-butanediol in Saccharomyces cerevisiae
Substrate channeling is a process of transferring an intermediate from one enzyme to the next enzyme without diffusion into the bulk phase, thereby leading to an enhanced reaction rate. Here, we newly designed substrate channeling modules in Saccharomyces cerevisiae based on a high affinity interaction between dockerin and cohesin domains, which is a key process in the formation of cellulosome ...

Transcriptome analyses to understand effects of the Fusarium deoxynivalenol and nivalenol mycotoxins on Escherichia coli
Fusarium spp. cause many diseases in farming systems and can produce diverse mycotoxins that can easily impact humans and animals through the ingestion of food and feed. Among these mycotoxins, deoxynivalenol (DON) and nivalenol (NIV) are considered the most important hazards because they can rapidly diffuse into cells and block eukaryotic ribosomes, leading to inhibition of the translation sys...
Comparative Evaluation of Fermentative Hydrogen Production using Enterobacter cloacae and Mixed Culture: Effect of Pd (II) ion and Phytogenic Palladium Nanoparticles
Palladium nanoparticles (PdNPs) were synthesized from PdCl2 using Coriandrum sativum leaf extract. The transmission electron microscopy (TEM) images confirm that the formation of PdNPs was mainly spherical in shape, with an average size of 87nm. The influence of the PdCl2 and synthesized PdNPs on fermentative hydrogen production from glucose using Enterobacter cloacae and mixed culture was eval...

Biorefining of wheat straw: accounting for the distribution of mineral elements in pretreated biomass by an extended pretreatment–severity equation
Mineral elements present in lignocellulosic biomass feedstocks may accumulate in biorefinery process streams and cause technological problems, or alternatively can be reaped for value addition. A better understanding of the distribution of minerals in biomass in response to pretreatment factors is therefore important in relation to development of new biorefinery processes. The objective of the ...
Feasibility of raw glycerol conversion into Single Cell Oil by Zygomycetes under non-aseptic conditions
The use of plant oils as feedstock for the biodiesel manufacture has many drawbacks, thus, the interest has turned to single cell oil (SCO) as an alternative. However, the production of SCO is still too expensive, mainly due to the low oil productivity and the high cost of medium sterilization required. In this work raw glycerol was converted into SCO by oleaginous Zygomycetes under non-aseptic...
Shear stress upregulates IL-1β secretion by Chlamydia pneumoniae- infected monocytes
Infectious agents are increasingly implicated in the development and progression of chronic inflammatory diseases. Several lines of evidence suggest that the common intracellular respiratory pathogen, Chlamydia pneumoniae contributes to the well-established risk factors of atherosclerosis but the exact mechanism is not well understood. It is believed that C. pneumoniae-infected monocytes travel...
Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites
We demonstrated the successful optimization of a recombinant multi-subunit malaria vaccine candidate protein for production in the methylotrophic yeast Pichia pastoris by the identification and subsequent removal of two protease cleavage sites. After observing protein degradation in the culture supernatant of a fed-batch fermentation, the predominant proteolytic fragment of the secreted recombi...