Biotechnology

AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation
Follistatin (FST) has been shown to bind to some TGF-? family members and can function as a potent myostatin (MSTN) antagonist. Recent studies have revealed that over-expression of FST by adeno-associated viruses increases muscle growth in mice, humans and nonhuman primates. In the present study, to determine the effect of FST on ovine primary myoblast (OPM) proliferation, FST was over-expresse...


Plant biotechnology for lignocellulosic biofuel production
Lignocelluloses from plant cell walls are attractive resources for sustainable biofuel production. However, conversion of lignocellulose to biofuel is more expensive than other current technologies, due to the costs of chemical pretreatment and enzyme hydrolysis for cell wall deconstruction. Recalcitrance of cell walls to deconstruction has been reduced in many plant species by modifying plant ...
Transgenic switchgrass (Panicum virgatum L.) biomass is increased by overexpression of switchgrass sucrose synthase ( PvSUS1)
Sucrose synthase converts sucrose and uridine di-phosphate (UDP) into UDP-glucose and fructose. UDP-glucose is used by the cellulose synthase to produce cellulose for cell wall biosynthesis. For lignocellulosic feedstocks such as switchgrass, the manipulation of cell walls to decrease lignin content is needed to reduce recalcitrance of conversion of biomass into biofuels. Of perhaps equal impor...


Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002
The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. Here we demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium, Synechococcus sp. PCC 7002. :Optimal growth conditions for co-...
Transition metal oxide catalysts as an alternative for the oxidation of nitrogen monoxide to nitrogen dioxide: kinetic modelling at high space velocity
The oxidation of NO to NO2 is a key step in environmental pollution abatement techniques, such as the “fast-SCR” or the diesel engines catalytic traps. In both cases, the conversion of an important fraction of NO into NO2 is a key step. In this work, two commercially available transition metal oxide catalyst, CuMn and CuCr-based, are studied as an alternative to noble metal catalysts (a Pt/...


Knockdown of EIF3D suppresses proliferation of human melanoma cells through G2/M phase arrest
Skin cancer is the most common malignancy with increasing incidence rates worldwide. The advanced form of skin cancer, melanoma, is resistant to conventional treatment methods, which puts a burden on researchers to identify alternative effective therapeutic approach. Herein, the current study was designed to identify the effects of si-RNA mediated silencing of EIF3D against melanoma cell surviv...
Adsorption Characteristics of Cellulase and β-glucosidase on Avicel, Pretreated Sugarcane Bagasse and Lignin
Although adsorption is an essential step in the enzymatic hydrolysis of lignocellulosic materials, literature reports controversial results in relation to the adsorption of the cellulolitic enzymes on different biomasses/pretreatments, which difficults the description of this phenomenon in hydrolysis mathematical models. In this work, the adsorption of these enzymes on Avicel and sugarcane baga...

Inhibition of protein phosphatase 5 (PP5) suppresses survival and growth of colorectal cancer cells
Protein phosphatase 5 (PP5) is a unique member of the PPs family that functions in multiple signaling pathways involved in DNA damage, cell cycle control, cell growth and apoptosis. Recent evidence indicated that PP5 may play a role in cancer progression. In this study, we aimed to examine the biological effect of PP5 on cell growth and apoptosis in human colorectal cancer. We firstly knocked d...
Biofunctionalized silver nanoparticles as a novel colorimetric probe for melamine detection in raw milk
Nanoparticles have emerged as a promising analytical tool for monitoring food adulteration and safety. In present study, silver nanoparticles (AgNPs) were synthesized using leaves extract of Jatropha gossypifolia. AgNPs revealed characteristic SPR peak at 419 nm and have spherical and grain shape with size range between 18-30 nm. A selective and rapid method of melamine detection in raw milk wa...

Production and characterization of thermostable alkaline protease of B. Subtilis (ATCC 6633) from optimized solid state fermentation
Proteases are the most important group of enzymes utilized commercially in various arenas of industries like food, detergent, leather, dairy, pharmaceutical, diagnostics, and waste management accounting for nearly 20% of the world enzyme market. Microorganism specially Bacillus genera serves as a vast repository of diverse set of industrially important enzymes and utilized for the large scale e...
KLF8 is required for bladder cancer cell proliferation and migration
Krüppel-like factor 8 (KLF8) belongs to the Sp/KLF family of transcription factors. Recently, it is affirmed that KLF8 plays an important role in the regulation of epithelial-mesenchymal transition (EMT), which is a key process occurs during cancer metastasis. Although the overexpression of KLF8 has been observed in several types of human cancers, the functional role of KLF8 in human bladder c...

Enhancing Functional Expression of Heterologous Proteins Through Random Substitution of Genetic Codes in the 5' Coding Region
Recent studies using heterologous protein expression systems suggest that synonymous codons affect not only the expression but also the properties of the expressed protein. However, practical application of this information is challenging, and to date, efforts to employ bioinformatics tools to design synonymous codon mixes have been only marginally successful. Here, we sought to enhance the fun...
Hydrolysis-Determining Substrate Characteristics in Liquid Hot Water Pretreated Hardwood
Fundamental characterization of pretreated hardwood and its interactions with cellulase enzymes has confirmed that a pathway exists for dramatically reducing the loading of cellulase required for hydrolysis of pretreated biomass. We demonstrate that addition of protein effecting a seven-fold decrease in the specific activity of cellulases enables a ten-fold reduction in enzyme loading while mai...
Microbial bio-based plastics from olive-mill wastewater: Generation and properties of polyhydroxyalkanoates from mixed cultures in a two-stage pilot scale system
The operational efficiency of a two stage pilot scale system for polyhydroxyalkanoates (PHAs) production from three phase olive oil mill wastewater (OMW) was investigated in this study. A mixed anaerobic, acidogenic culture derived from a municipal wastewater treatment plant, was used in the first stage, aiming to the acidification of OMW. The effluent of the first bioreactor that was operated ...
The use of a combined respirometric – titrimetric setup to assess the effect of environmental conditions on micro – algal growth rate
Due to their complexity a lot of research is performed on micro-algal systems for wastewater treatment. In this work a previously developed combined respirometric – titrimetric approach was used for the determination of micro - algal kinetics. A Plackett- Burman design with fold-over was executed to investigate the influence of 8 different degrees of freedom regarding environmental conditions...