Biotechnology

The transcription factor Ap-1 regulates monkey 20alpha-hydroxysteroid dehydrogenase promoter activity in CHO cells
Monkey 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) is a catabolic enzyme responsible for converting progesterone into biologically inactive 20alpha-hydroxyprogesterone, thereby playing a key role in the estrous cycle or pregnancy and allowing ovulation and parturition to occur in most mammalian animals. Monkey 20alpha-HSD was highly abundant in ovarian and placental tissues during the pr...


The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis
In human vaccine manufacturing some pathogens such as Modified Vaccinia Virus Ankara, measles, mumps virus as well as influenza viruses are still produced on primary material derived from embryonated chicken eggs. Processes depending on primary cell culture, however, are difficult to adapt to modern vaccine production. Therefore, we derived previously a continuous suspension cell line, AGE1. CR...
A novel scaffold geometry for chondral applications: Theoretical model and in vivo validation
A theoretical model of the 3D scaffold internal architecture has been implemented with the aim to predict the effects of some geometrical parameters on total porosity, Young modulus, buckling resistance and permeability of the graft. This model has been adopted to produce porous poly-caprolacton based grafts for chondral tissue engineering applications, best tuning mechanical and functional fea...


De Novo Characterisation of the Greenlip Abalone Transcriptome (Haliotis laevigata) with a Focus on the Heat Shock Protein 70 (HSP70) Family
Abalone (Haliotis) are economically important molluscs for fisheries and aquaculture industries worldwide. Despite this, genomic resources for abalone and molluscs are still limited. Here we present a description and functional annotation of the greenlip abalone (Haliotis laevigata) transcriptome. We present a focused analysis on the heat shock protein 70 (HSP70) family of genes with putative f...
High throughput platform to explore RNA–protein interactomes
Rna binding proteins (RBPs) and rna interaction is an emerging topic in molecular biology. Many reports showed that such interactions contribute to many cellular processes as well as disease development. Several standard in vitro and in vivo methods were developed to fulfill the needs of this RBP–RNA interaction study to explore their biological functions. However, these methods have their li...


Agave biotechnology: an overview
Agaves are plants of importance both in Mexican culture and economy and in other Latin-American countries. Mexico is reported to be the place of Agave origin, where today, scientists are looking for different industrial applications without compromising its sustainability and preserving the environment. To make it possible, a deep knowledge of all aspects involved in production process, agro-ec...
Enhanced production of xylanase by solid state fermentation using Trichoderma koeningi isolate: effect of pretreated agro-residues
The main objective of this study was to isolate the fungal strain for enhanced production of xylanase using different agro-residues and fruit peels by solid state fermentation and its potentiality was tested on the pretreated corn cob. Fermentation was carried out with Trichoderma koeningi isolate using untreated and pretreated corn cob supplemented with pineapple peel powder showed higher prod...

Genome sequence of Mycobacterium aromaticivorans JS19b1T, a novel isolate from Hawaiian soil
Mycobacterium aromaticivorans JS19b1T (=ATCC BAA-1378T, DSM 45407T), isolated from petroleum-contaminated Hawaiian soil, has received much attention by possessing the powerful degrading capacity to polycyclic aromatic compounds (PAHs). With a significance of first genome sequence for M. aromaticivorans, the draft genome sequence of strain JS19b1T described in this study can provide the genomic ...
Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing
The market development of plasmid biopharmaceuticals for gene therapy and DNA vaccination applications is critically dependent on the availability of cost-effective manufacturing processes capable of delivering large amounts of high-quality plasmid DNA (pDNA) for clinical trials and commercialization. The producer host strain used in these processes must be designed to meet the upstream and dow...

Improvement and simplification of fed-batch bioprocesses with a highly soluble phosphotyrosine sodium salt
Fed-batch culture bioprocesses are currently used predominantly for the production of recombinant proteins, especially monoclonal antibodies. In these cultures, concentrated feeds are added during cultivation to prevent nutrient depletion, thus extending the cellular growth phase and increasing product concentrations. One limitation in these bioprocesses arises from the low solubility or stabil...
Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody
The impact of operating conditions on the glycosylation pattern of humanized camelid monoclonal antibody, EG2-hFc produced by Chinese hamster ovary (CHO) cells has been evaluated by a combination of experiments and modeling. Cells were cultivated under different levels of glucose and glutamine concentrations with the goal of investigating the effect of nutrient depletion levels and ammonia buil...

Heterologous pathway for the production of l-phenylglycine from glucose by E. coli
The aproteinogenic amino acid l-phenylglycine (l-Phg) is an important side chain building block for the preparation of several antibiotics and taxol. To biosynthesis l-Phg from glucose, an engineered Escherichia coli containing l-Phg synthetic genes was firstly developed by an l-phenylalanine producing chassis supplying phenylpyruvate. The enzymes HmaS (l-4-hydroxymandelate synthase), Hmo (l-4-...
Porcine parvovirus flocculation and removal in the presence of osmolytes
Viruses can be modified into viral vaccines or gene therapy vectors in order to treat acquired or genetic diseases. To satisfy the current market demand, an improvement in current vaccine manufacturing is needed. Chromatography and nanofiltration are not suitable for all types of viruses. In this study, we propose to use virus flocculation with osmolytes, followed by microfiltration, as a poten...
Versatile and stable vectors for efficient gene expression in Ralstonia eutropha H16
The Gram-negative β-proteobacterium Ralstonia eutropha H16 is primarily known for polyhydroxybutyrate (PHB) production and its ability to grow chemolithoautotrophically by using CO2 and H2 as sole carbon and energy sources. The majority of metabolic engineering and heterologous expression studies conducted so far rely on a small number of suitable expression systems. Particularly the plasmid b...
Inhibition of biofilm formation by Camelid single-domain antibodies against the flagellum of Pseudomonas aeruginosa
Pseudomonas aeruginosa is a leading cause of hospital-acquired infections in patients with compromised host defense mechanisms, including burn wound victims. In addition to its intrinsic resistance against most antibiotics, P. aeruginosa has the ability to form biofilms adhering to biotic or abiotic surfaces. These factors make treatment of P. aeruginosa infections complicated and demand new th...