Biotechnology

Reactive extraction of amino acids mixture in hydrolysate from cottonseed meal with di(2-ethylhexyl) phosphoric acid
In recent years there has been a noticeable growth of amino acid utilization throughout the world. How to separate and purify amino acids from fermentation broths or protein hydrolysates in an effective and low cost way is increasingly becoming an urgent problem. Utilization of solvent extraction technology in amino acids separation field brings forward a solution. Extractions of individual ami...


Bioactive compounds and advanced processing technology: Phaleria macrocarpa (sheff.) Boerl, a review
Recent technological advances and the development of new methods has provided an opportunity to obtain highly purified natural bioactive compounds extracts with potential for the treatment and prevention of human diseases. The use of hazardous and toxic solvents in extraction and processing of bioactive compounds from plant materials is considered as a big problem in health, safety and environm...
Cloning and Characterization of an Inulinase Gene From the Marine Yeast Candida membranifaciens subsp. flavinogenie W14-3 and Its Expression in Saccharomyces sp. W0 for Ethanol Production
The INU1 gene encoding an exo-inulinase from the marine-derived yeast Candida membranifaciens subsp. flavinogenie W14-3 was cloned and characterized. It had an open reading frame of 1,536 bp long encoding an inulinase. The coding region of it was not interrupted by any intron. The cloned gene encoded 512 amino acid residues of a protein with a putative signal peptide of 23 amino acids and a ca...


In planta Production of Flock House Virus Transencapsidated RNA and Its Potential Use as a Vaccine
We have developed a transencapsidated vaccine delivery system based on the insect virus, Flock House virus (FHV). FHV is attractive due to its small genome size, simple organization, and nonpathogenic characteristics. With the insertion of a Tobacco mosaic virus (TMV) origin of assembly (Oa), the independently replicating FHV RNA1 can be transencapsidated by TMV coat protein. In this study, we ...
RNAi-based GM plants: food for thought for risk assessors
RNA interference (RNAi) is an emerging technology that offers new opportunities for the generation of new traits in genetically modified (GM) plants. Potential risks associated with RNAi-based GM plants and issues specific to their risk assessment were discussed during an international scientific workshop (June 2014) organized by the European Food Safety Authority (EFSA). Selected key outcomes ...


Rice transgene flow: its patterns, model and risk management
Progress has been made in a 12 year's systemic study on the rice transgene flow including (i) with experiments conducted at multiple locations and years using up to 21 pollen recipients, we have elucidated the patterns of transgene flow to different types of rice. The frequency to male sterile lines is 101 and 103 higher than that to O. rufipogon and rice cultivars. Wind speed and direction a...
Modifying plants for biofuel and biomaterial production
The productivity of plants as biofuel or biomaterial crops is established by both the yield of plant biomass per unit area of land and the efficiency of conversion of the biomass to biofuel. Higher yielding biofuel crops with increased conversion efficiencies allow production on a smaller land footprint minimizing competition with agriculture for food production and biodiversity conservation. P...

Transient expression assays in grapevine: a step towards genetic improvement
In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced many gene data sets that require detailed analysis. Due to their rapid nature, tr...
Understanding and engineering beneficial plant–microbe interactions: plant growth promotion in energy crops
Plant production systems globally must be optimized to produce stable high yields from limited land under changing and variable climates. Demands for food, animal feed, and feedstocks for bioenergy and biorefining applications, are increasing with population growth, urbanization and affluence. Low-input, sustainable, alternatives to petrochemical-derived fertilizers and pesticides are required ...

Production of novel biopolymers in plants: recent technological advances and future prospects
The production of novel biopolymers in plants has the potential to provide renewable sources of industrial materials through agriculture. In this review we will highlight recent progress with plant-based production of polyhydroxyalkanoates (PHAs), silk, elastin, collagen, and cyanophycin with an emphasis on the synthesis of poly[(R)-3-hydroxybutyrate] (PHB), a renewable biodegradable PHA polyme...
How metabolites modulate metabolic flux
Adaptation to metabolic needs and changing environments is a basic requirement of every living system. These adaptations can be very quick and mild or slower but more drastic. In any case, cells have to constantly monitor their metabolic state and requirements. In this article we review general concepts as well as recent advances on how metabolites can regulate metabolic fluxes. We discuss how ...

Controlled processing of a full-sized porcine liver to a decellularized matrix in 24 h
The generation of full-sized humanized organs based on animal matrix scaffolds is a promising approach to overcome the shortage of transplant organs. Recent decellularization methods are mostly time-consuming and associated with large rinsing volumes and poorly standardized procedures. In this study we developed an optimized rapid and standardized decellularization method to obtain a functional...
Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblasts
Skeletal muscle regeneration requires migration, proliferation and fusion of myoblasts to form multinucleated myotubes. In our previous study, we showed that insulin-like growth factor (IGF)-I gene delivery stimulates the proliferation and differentiation of mouse myoblast C2C12 cells and promotes the contractile force generated by tissue-engineered skeletal muscles. The aim of this study was t...
Maintenance of an undifferentiated state of human induced pluripotent stem cells through migration-dependent regulation of the balance between cell–cell and cell–substrate interactions
We present an outlook on the current strategies for maintaining and culturing human induced pluripotent stem cells (hiPSCs) in an undifferentiated state without affecting their pluripotency. First, cell structures and function are described in relation to interactions between hiPSCs and their surroundings. Second, the phenomenon of spontaneous deviation from undifferentiated hiPSCs in cultures ...
Characterization of two amine oxidases from Aspergillus carbonarius AIU 205
We have reported that Aspergillus carbonarius AIU 205, which was isolated by our group, produced three enzymes exhibiting oxidase activity for 4-aminobutanamide (4-ABAD) (J. Biosci. Bioeng., 117, 263–268 (2014)). Among three enzymes, characteristics of enzyme I have been revealed, but those of the other two enzymes have not. In this study, we purified enzymes II and III, and compared their ch...