Biotechnology

Standardization of medium composition and agricultural waste in the production of p -hydroxybenzoic acid by Paecilomyces variotii
Biotransformation of p-coumaric acid by Paecilomyces variotii resulted in the accumulation of a metabolite which was identified as p-hydroxybenzoic acid. We studied the possibility of enhancing this metabolite by various parameters such as varying the concentration of substrate, addition of different nitrogen sources and carbon sources at varying concentration and use of different agricultural ...


Differential Proteomics Analysis in Sunflower (Helianthus annuus L.)
The seed proteins of sunflower (Helianthus annuus) were qualitatively and quantitatively investigated. Qualitative studies were carried out using different electrophoretic techniques (2-DE). Analysis of the water extracted flour on SDS-PAGE gave eight major polypeptides clusters with MWs of lower than 14.4, 14.4-18.4, 18.4-25.0, 25-35, 35-45, 45-66.2, 66.2-116 and higher than 116 kDa. Second di...
Encapsulation of Biosurfactant-Producing Bacillus licheniformis (PTCC 1320) in Alginate Beads
In the present study, biosurfactant production by immobilized Bacillus licheniformis PTCC 1320 in alginate beads has been studied. Alginate solution and bacterial suspension were mixed and stirred to get a uniform mixture. The slurry was taken into a sterile syringe and added drop wise into a stirring calcium chloride solution from a 5 cm height to form spherical beads. The beads were kept unde...


Protein Profile Expression of Clarias gariepinus, Heterobranchus bidorsalis and their Reciprocal Hybrids in South-West Nigeria
Proper genetic characterization would help in the selection of suitable strains for aquaculture that could lead to production of new varieties of fishes to alleviate the problem of short supply of fast growing quality fish seeds. The study was aimed at analyzing the muscle protein profiles of Clarias gariepinus, Heterobranchus bidorsalis and their reciprocal hybrids. Sixteen juveniles fish samp...
Kernel-Based Informative Metric for Morphological Differences in Cell Images
The aim of this study is to propose a framework for Quantification and Calibration of Morphological Differences (QCMD) to extract nonlinear features. The framework based on our proposed Kernel informative energy which extends previous energy-based approaches in several important directions. First while previous research has defined Graph transition energy to quantify the similarity between coll...


Salicylic Acid and Acquisition of Desiccation Tolerance in Pisum sativum Seeds
Desiccation tolerance in orthodox seeds is acquired during various phases of development and lost upon imbibition/germination. In this study, germinated seeds of pea (Pisum sativum) with a protruded radicle length of 10 mm did not survive desiccation below 0.43 g H2O g-1. The re-establishment/resumption of radicle growth after desiccation were achieved under both Mili-Q water and salicylic acid...
Differences in Mannose-Binding Lectin Gene Polymorphisms in Different Diseases
Mannose-Binding Lectin (MBL) is a member of the collectin family. It binds to various oligosaccharides and activates the classical pathway of complement independent from C1q. The aim of present study is to study the distribution of the alleles of MBL gene and promoter variants in intracellular, extracellular pathogens and autoimmune diseases. Our studies showed occurrence of the codon 54 mutati...

Production, Purification and Application of Bacterial Laccase: A Review
Laccases are versatile enzymes belonging to the group of oxidases. Laccases catalyzes variety of phenolic compounds, as well as, diamines and aromatic amines with concomitant reduction of molecular oxygen to water. Laccases have mostly been isolated and characterized from plants and fungi in contrast, little is known about bacterial laccases. Thermostability of this particular enzyme makes an a...
Reactor concepts for bioelectrochemical syntheses and energy conversion
In bioelectrochemical systems (BESs) at least one electrode reaction is catalyzed by microorganisms or isolated enzymes. One of the existing challenges for BESs is shifting the technology towards industrial use and engineering reactor systems at adequate scales. Due to the fact that most BESs are usually deployed in the production of large-volume but low-value products (e.g., energy, fuels, and...

Development and Utilization of a Custom PCR Array Workflow: Analysis of Gene Expression in Mycoplasma genitalium and Guinea Pig ( Cavia porcellus )
Transcriptome analysis is a powerful tool for evaluating molecular pathways central to maturation of specific biological processes and disease states. Recently, PCR-based arrays have supplemented microarray and RNA-seq methodologies for studying changes in gene expression levels. PCR arrays are a more cost efficient alternative, however commercially available assemblies are generally limited to...
Bioconversion of tea polyphenols to bioactive theabrownins by Aspergillus fumigatus
Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state Pu-erh tea fermentation. A theabrownins yield of 158 g kg−1 sun-dried gre...

Development of genetic transformation methodologies for an industrially-promising microalga: Scenedesmus almeriensis
The development of the microalgal industry requires advances in every aspect of microalgal biotechnology. In this regard, the availability of genetic engineering tools for industrially-promising species is key. As Scenedesmus almeriensis has promise for industrial use, we describe here an Agrobacterium-based methodology that allows stable genetic transformation of it for the first time, thus op...
One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
Yarrowia lipolytica is an unconventional yeast, and is generally recognized as safe (GRAS). It provides a versatile fermentation platform that is used commercially to produce many added-value products. Here we report a multiple fragment assembly method that allows one-step integration of an entire β-carotene biosynthesis pathway (~11 kb, consisting of four genes) via in vivo homologous recomb...
Protoplast fusion enhances lignocellulolytic enzyme activities in Trichoderma reesei
Protoplast fusion was used to obtain a higher production of lignocellulolytic enzymes with protoplast fusion in Trichoderma reesei. The fusant strain T. reesei JL6 was obtained from protoplast fusion from T. reesei strains QM9414, MCG77, and Rut C-30. Filter paper activity of T. reesei JL6 increased by 18 % compared with that of Rut C-30. β-Glucosidase, hemicellulase and pectinase activities ...
Regioselective hydroxylation of 17β-estradiol by mutants of CYP102A1 from Bacillus megaterium
A large set of mutants of CYP102A1 from Bacillus megaterium have human cytochrome P450-like activities and the ability to metabolize a number of marketed drugs and steroids. Here, we tested whether the CYP102A1 mutants could be used to produce hydroxylated human metabolites of 17β-estradiol (E2). A set of the mutants, which were generated by site-directed and random mutagenesis, was used to pr...