Biotechnology

The biophysical, biochemical, and biological toolbox for tenogenic phenotype maintenance in vitro
Tendon injuries constitute an unmet clinical need, with 3 to 5 million new incidents occurring annually worldwide. Tissue grafting and biomaterial-based approaches fail to provide environments that are conducive to regeneration; instead they lead to nonspecific cell adhesion and scar tissue formation, which collectively impair functionality. Cell based therapies may potentially recover native t...


Genetic Mapping and QTL Analysis of Growth Traits in the Large Yellow Croaker Larimichthys crocea
Large yellow croaker (Larimichthys crocea) is an important maricultured species in China. A genetic linkage map of the large yellow croaker was constructed using type II microsatellites and expressed sequence tag (EST)-derived microsatellites in two half-sib families (two females and one male). A total of 289 microsatellite markers (contained 93 EST-SSRs) were integrated into 24 linkage groups,...
Supercritical water gasification of biomass in diamond anvil cells and fluidized beds
With the shift in interest towards renewable energy, hydrogen as an alternative gaseous fuel seems to attract much attention. Waste biomass is an ideal option for the synthesis of biofuels due to its abundance and no net CO2 emissions. Hydrogen can be produced through supercritical water gasification of waste biomass. Hydrogen is an attractive energy carrier which can be used as a direct fuel o...


From molecular evolution to biobricks and synthetic modules: a lesson by the bacterial flagellum
The bacterial flagellum is a motility structure and represents one of the most sophisticated nanomachines in the biosphere. Here, we review the current knowledge on the flagellum, its architecture with respect to differences between Gram-negative and Gram-positive bacteria and other species-specific variations (e.g. the flagellar filament protein, Flagellin). We further focus on the mechanism b...
SiRNA: novel therapeutics from functional genomics
Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siRNA-mediated silencing and finally siRNA has emerged as a powerful therapeutic tool. With siRNAs virtually every gene in the human genome contributing to a disease becomes amenable to regulation, thus opening unprecedented opportunities for drug discovery. siRNA has a well-established role as a tool ...


Does nature make provision for backups in the modification of bacterial community structures?
Self-balancing is an inherent character in nature in response to community structure modification pressure and modern biotechnology has revolutionized the way such detections are made. Presented here is an overview of the forces and process interactions between released bacteria and indigenous microflora which encompass soil bacterial diversity, community structure, indigenous endorhizosphere m...
Molecular cloning, relative expression and structural analysis of pattern recognition molecule β-glucan binding protein from mangrove crab Episesarma tetragonum
A full length cDNA of a β-GBP gene was identified from the mangrove crab Episesarma tetragonum. The open reading frame (ORF) of the E. tetragonum β-GBP (Epte β-GBP) was 1167 bp long, encoding a polypeptide of 389 amino acids. The deduced amino acid sequence of Epte β-GBP gene has conserved potential recognition motif for β-1, 3 linkages of polysaccharides and putative RGD (Arg–Gly–Asp)...

Split Ssp DnaB Mini-intein Mediated Production of Recombinant Human Glucagon Like Peptide-1/7-36
Glucagon like peptide-1 plays an important role in the regulation of postprandial insulin release. Here we used the split DnaB mini-intein system to produce recombinant human GLP-1/7–36 (rhGLP-1) in Escherichia coli. The C-terminal domain of DnaB mini-intein (IntC) was genetically fused at the N-terminus of rhGLP-1 to produce IntC-GLP-IntC-GLP-1 and N-terminal domain of DnaB mini-intein (IntN...
Assembly and Multiple Gene Expression of Thermophilic Enzymes in Escherichia coli for In Vitro Metabolic Engineering
In vitro reconstitution of an artificial metabolic pathway is an emerging approach for the biocatalytic production of industrial chemicals. However, several enzymes have to be separately prepared (and purified) for the construction of an in vitro metabolic pathway, thereby limiting the practical applicability of this approach. In this study, genes encoding the nine thermophilic enzymes involved...

Effect of anion structures on cholinium ionic liquids pretreatment of rice straw and the subsequent enzymatic hydrolysis
In this work, 28 cholinium ILs, most of which are good solvents for dissolving lignin, were used for rice straw pretreatment to improve subsequent enzymatic hydrolysis. The anion exerted a significant effect on the pretreatment effectiveness of the IL as well as the subsequent enzymatic hydrolysis efficiency of rice straw residues. The presence of the basic group(s) in the anion significantly e...
Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro
This study is aimed at investigating the most efficient and cost effective sterilization condition for initiation of sweet potato culture in-vitro. Different concentrations of common or local household bleach (Power zone and Dettol) and non-readily available mercuric chloride as well as variation in their duration for sterilization were used to sterilize sweet potato buds to study their effecti...

Sulforaphane down-regulates SKP2 to stabilize p27KIP1 for inducing antiproliferation in human colon adenocarcinoma cells
Sulforaphane is a cruciferous vegetable-derived isothiocyanate with promising chemopreventive and therapeutic activities. Induction of proliferation arrest and apoptosis principally contribute to sulforaphane's anticancer activity, but the precise molecular mechanisms remain elusive. The oncoprotein SKP2 is a key component of the SKP1-CULLIN1-F-box (SCF) E3 ligase complex and is responsible for...
Autotrophic denitrification performance and bacterial community at biocathodes of bioelectrochemical systems with either abiotic or biotic anodes
Two-chamber bioelectrochemical systems (BESs) have recently been developed for nitrate removal from nitrate-contaminated water. In this study, we compared the nitrate removal performance of biocathodes of BESs when using abiotic and biotic anodes. Acetate was used as electron donor in BESs with biotic anode, whereas a direct current power supply was used as energy source in BESs with abiotic an...
Inside industry
Sanford-Burnham and Daiichi Sankyo form alliance to study novel drug targets in cardiovascular metabolic diseases. Sigma-Aldrich® to launch Austrianova's Cell-in-a-Box® Kit for research use. ASLAN Pharmaceuticals signs agreement with CSL to develop novel therapy for asthma. Self-assembling peptides for bone regeneration and drug delivery. Verisante Technology, Inc. receives the 2014 North Ame...
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Translating Research at SingHealth: Cancer. Translating Research at SingHealth: Cardiometabollic Diseases. Translating Research at SingHealth: Eye Diseases. Translating Research at SingHealth: Health Services Research. Translating Research at SingHealth: Infectious Diseases. Translating Research at SingHealth: Neurosciences (Stroke).