Biotechnology

Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species
The γ-tocopherol methyltransferase (γ-TMT) is an important enzyme regulating synthesis of four tocopherols (α, γ, β and δ). In this report, we investigated the role of γ-TMT in regulating abiotic stress within chloroplasts. The At γ-tmt overexpressed via the tobacco chloroplast genome accumulated up to 7.7% of the total leaf protein, resulting in massive proliferation of the inner envel...


Modulation of amylose content by structure-based modification of OsGBSS1 activity in rice (Oryza sativa L.)
The rice Waxy (Wx) gene encodes granule-bound starch synthase 1 (EC 2.4.1.242), OsGBSS1, which is responsible for amylose synthesis in rice seed endosperm. In this study, we determined the functional contribution of eight amino acids on the activity of OsGBSS1 by introducing site-directed mutated Wx gene constructs into the wx mutant glutinous rice. The eight amino acid residues are suspected t...
Identification and characterization of promoters specifically and strongly expressed in maize embryos
The use of maize seeds as bioreactors has several advantages for the production of recombinant proteins in plant biotechnology, but available embryo-specific and strong promoters are limited. Here, we describe a genome-scale microarray-based approach to identify embryo-specifically and strongly expressed genes and their promoters in maize. We identified 28 embryo-preferred and abundantly expres...


The Bromotyrosine Derivative Ianthelline Isolated from the Arctic Marine Sponge Stryphnus fortis Inhibits Marine Micro- and Macrobiofouling
The inhibition of marine biofouling by the bromotyrosine derivative ianthelline, isolated from the Arctic marine sponge Stryphnus fortis, is described. All major stages of the fouling process are investigated. The effect of ianthelline on adhesion and growth of marine bacteria and microalgae is tested to investigate its influence on the initial microfouling process comparing with the known mari...
RNA-Binding Proteins Associated Molecular Mechanisms of Motor Neuron Degeneration Pathogenesis
Motor neuron diseases are neurodegenerative disorders that trigger motor neurons to degenerate and lead to paralysis. Our understanding on the mechanisms of motor neuron degeneration has been enhanced by recent cellular and molecular researches. In this review, I highlight advances in RNA-binding proteins associated molecular mechanisms of motor neuron degeneration pathogenesis including ribonu...


The Electrochemical Production of Boric Acid
Boric acid (H3BO3) was electrochemically produced from borax pentahydrate (Na2B4O7.5H2O) using membrane electrolysis and flow diagram of the production process was developed. The effect of different catholyte concentrations (10, 20 and 30 % aqueous NaOH) and current densities (1.2, 1.8 and 2.9 kA/m2) on the current efficiency was studied. The electrical power consumption per ton for the product...
Microwave-assisted heterogeneous catalytic oxidation of high-concentration Reactive yellow 3 with CuFe2O4/PAC
The investigation of microwave-assisted heterogeneous catalytic oxidation on high-concentration dye effluent with novel catalyst (CuFe2O4/PAC) is promising in the field of industrial wastewater treatment compared with conventional advanced oxidation technique which consumed long time. A novel microwave catalyst, CuFe2O4 loaded on powdery activated carbon (CuFe2O4/PAC) was prepared and applied i...

Lignin Solubility in Non-Imidazolium Ionic Liquids
Up till now ionic liquid research concerning lignin dissolution and processing has mainly focused on the imidazolium type ionic liquids. Other types of ionic liquids can extend the scope of such processes due to their better thermal, base and oxidation stability. A series of ammonium, phosphonium and pyrrolidinium based ionic liquids was screened for lignin dissolution. Tributylmethylphosphoniu...
Application of High-Temperature Fenton Oxidation for the Treatment of Sulfonation Plant Wastewater
This work explores the application of intensified Fenton process to a sulfonation plant wastewater for its further discharge into an activated sludge plant. The application of high-temperature Fenton to this kind of wastewater has not been reported so far. Herein, it has been demonstrated to provide a better performance than other AOPs. The factorial experimental design methodology applied for ...

Isolation and characterization of awamori yeast mutants with l-leucine accumulation that overproduce isoamyl alcohol
Awamori shochu is a traditional distilled alcoholic beverage made from steamed rice in Okinawa, Japan. Although it has a unique aroma that is distinguishable from that of other types of shochu, no studies have been reported on the breeding of awamori yeasts. In yeast, isoamyl alcohol (i-AmOH), known as the key flavor of bread, is mainly produced from α-ketoisocaproate in the pathway of l-leuci...
Glycerol and neutral lipid production in the oleaginous marine diatom Phaeodactylum tricornutum promoted by overexpression of glycerol-3-phosphate dehydrogenase
Microalgae are ideal raw materials for biodiesel and bioactive compounds. Glycerol-3-phosphate is formed from dihydroxyacetone phosphate (DHAP) through the glycolytic pathway catalyzed by glycerol-3-phosphate dehydrogenase (GPDH). :GPDH was characterized in the marine diatom Phaeodactylum tricornutum. In the GPDH-overexpressing P. tricornutum cells, the glycerol concentration per cell in the tr...

Gluten quality of bread wheat is associated with activity of RabD GTPases
In the developing endosperm of bread wheat (Triticum aestivum), seed storage proteins are produced on the rough endoplasmic reticulum (ER) and transported to protein bodies, specialized vacuoles for the storage of protein. The functionally important gluten proteins of wheat are transported by two distinct routes to the protein bodies where they are stored: vesicles that bud directly off the ER ...
Re-assessment of
Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was perf...
Six New Vibralactone Derivatives from Cultures of the Fungus Boreostereum vibrans
Phytochemical reinvestigation on the cultural broth of Boreostereum vibrans led to the isolation of six new vibralactone derivatives, vibralactone N (1), vibralactone O (2), vibralactone P (3), 10-lactyl vibralactone G (4), (3S*, 4R*)-6-acetoxymethyl-2,2-dimethyl-3,4-dihydro-2H-chromene-3,4-diol (5), vibralactone Q (6). Their structures were elucidated by extensive spectroscopic methods....
Highly Oxygenated Limonoids and Lignans from Phyllanthus flexuosus
Two new highly oxygenated limonoids, flexuosoids A (1) and B (2), and three new arylnaphthalene lignan glycosides, phyllanthusmins D–F (3–5), were isolated from the roots of Phyllanthus flexuosus, in addition to three known lignans, phyllanthusmin C, arabelline, and (+)-diasyringaresinol. Their structures were elucidated on the basis of detailed spectroscopic analysis and chemical methods. ...