Biotechnology

Solid fermentation of wheat bran for hydrolytic enzymes production and saccharification content by a local isolate Bacillus megatherium
Back ground: For enzyme production, the costs of solid state fermentation (SSF) techniques were lower and the production higher than submerged cultures. A large number of fungal species was known to grow well on moist substrates, whereas many bacteria were unable to grow under this condition. Therefore, the aim of this study was to isolate a highly efficient strain of Bacillus sp utilizing whea...


A new insect cell glycoengineering approach provides baculovirus-inducible glycogene expression and increases human-type glycosylation efficiency
Insect cells are often glycoengineered using DNA constructs encoding foreign glyocoenzymes under the transcriptional control of the baculovirus immediate early promoter, ie1. However, we recently found that the delayed early baculovirus promoter, 39 K, provides inducible and higher levels of transgene expression than ie1 after baculovirus infection. Thus, the purpose of this study was to asses...
Prediction of IgG1 aggregation in solution
Interest in monoclonal antibody aggregation is increasing as aggregates of biopharma-ceuticals can cause an immunogenic response when injected into the body. In this work a stoichiometric reaction model from concentration-time data is developed to predict the dimer ratio in stored antibody solutions over time. IgG1 was incubated at pH from 4.5 to 5.5, salt concentrations from 100 to 600 mmol/kg...


Methodology for biorefinery portfolio assessment using supply-chain fundamentals of bioproducts
Given the emergence of innovative processes in recent years for the manufacture of bioproducts from second-generation biomass, a range of unique biorefinery strategies are likely to be implemented by forest product companies in the coming years. No matter what biorefinery strategy is employed, to compete in the longer term, it will be critical to have a supply-chain adapted to the targeted prod...
New process for sustainable fuels and chemicals from bio-based alcohols and acetone
Synthesizing complex and large molecules out of small molecules derived by fermentation processes is a key factor for future processes based on renewable resources. Promising steps are the self-condensation of alcohols and the cross-condensation of alcohols with acetone. The presented continuous heterogeneous-catalytic gas-phase process makes it possible to condense small alcohols and ketones t...


Technical guide for genetic advancement of underdeveloped and intractable Clostridium
In recent years, the genus Clostridium has risen to the forefront of both medical and industrial biotechnology owing to its potential in applications as diverse as anticancer therapy and production of commodity chemicals and biofuels. The prevalence of hyper-virulent strains of C. difficile within medical institutions has also led to a global epidemic that demands a more thorough understanding ...
People watch
Asia-Pacific Biotech News, Volume 18, Issue 03, Page 47-49, March 2014. Providing for Emerging Markets: A Chat with Raman Singh from Mundipharma.

Physiological effects of over-expressing compartment-specific components of the protein folding machinery in xylose-fermenting Saccharomyces cerevisiae
Efficient utilization of both glucose and xylose is necessary for a competitive ethanol production from lignocellulosic materials. Although many advances have been made in the development of xylose-fermenting strains of Saccharomyces cerevisiae, the productivity remains much lower compared to glucose. Previous transcriptional analyses of recombinant xylose-fermenting strains have mainly focused...
A review of biological delignification and detoxification methods for lignocellulosic bioethanol production
Future biorefineries will integrate biomass conversion processes to produce fuels, power, heat and value-added chemicals. Due to its low price and wide distribution, lignocellulosic biomass is expected to play an important role toward this goal. Regarding renewable biofuel production, bioethanol from lignocellulosic feedstocks is considered the most feasible option for fossil fuels replacement ...

Engineering Saccharomyces cerevisiae for direct conversion of raw, uncooked or granular starch to ethanol
The production of raw starch-degrading amylases by recombinant Saccharomyces cerevisiae provides opportunities for the direct hydrolysis and fermentation of raw starch to ethanol without cooking or exogenous enzyme addition. Such a consolidated bioprocess (CBP) for raw starch fermentation will substantially reduce costs associated with energy usage and commercial granular starch hydrolyzing (GS...
Biologically synthesized silver nanoparticles induce neuronal differentiation of SH-SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathwayss
The relevant in vitro cellular model resembling functional neurons is important for the mechanistic research on various neuronal diseases. Human neuroblastoma SH-SY5Y cells may be considered one of the ideal in vitro models for studying neuroscience, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron-like phenotypes in vitro by several differentiat...

Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media
The efficiency and productivity of cellular biocatalysts play a key role in the industrial synthesis of fine and bulk chemicals. This study focuses on optimizing the synthesis of (S)-limonene from glycerol and glucose as carbon sources using recombinant E. coli. The cyclic monoterpene limonene is extensively used in the fragrance, food, and cosmetic industries. Recently, limonene also gained in...
Opportunities and challenges for seaweed in the biobased economy
The unique chemical composition of seaweeds and their fast growth rates offer many opportunities for biorefining. In this article we argue that cascading biorefinery valorization concepts are viable alternatives to only using seaweeds as carbohydrate sources for the fermentative production of biofuels. However, many challenges remain with respect to use of seaweeds for chemical production, such...
Caution required for handling genome editing technology
Genome-editing technology, although a robust tool for genetic engineering, is creating indistinct regulatory boundaries between naturally occurring and modified organisms. However, researchers must act with caution in research and development to avoid misleading society. Furthermore, appropriate regulations should be proactively discussed and established for handling genome-editing technology.
Two Natural ent-kauranoids as Novel Wnt Signaling Inhibitors
Constitutively active Wnt signaling frequently occurs in most colon cancers. Therefore, inhibitors of Wnt signaling pathway could provide rational therapeutic effects for colorectal malignancy. Within this paper, we identified two inhibitors of Wnt signaling pathway, rabdoternin B and maoecrystal I from a natural ent-kauranoid library by a dual-luciferase reporter gene assay. The two compounds ...