Biotechnology

Overexpression of Horsegram (Macrotyloma uniflorum Lam.Verdc.) NAC Transcriptional Factor (MuNAC4) in Groundnut Confers Enhanced Drought Tolerance
The NAC family being the largest plant-specific transcription factors functions in diverse and vital physiological processes during development. NAC proteins are known to be crucial in imparting tolerance to plants against abiotic stresses, such as drought and salinity, but the functions of most of them are still elusive. In this study, we report for the first time expression of the MuNAC4, a m...


Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase
Two α-1,3-glucanase isozymes, designated as α-1,3-glucanase 1 (Agl-FH1) and α-1,3-glucanase 2 (Agl-FH2), were purified from the culture medium of Paenibacillus glycanilyticus FH1Agl-FH1 and Agl-FH2 exhibited similar characteristics such as optimal pH, pH stability, optimal temperature, thermostability, and molecular masses on SDS-PAGE. However, their hydrolysis products of α-1,3-glucan vari...
Genomic profile of the plants with pharmaceutical value
There is an ample genetic diversity of plants with medicinal importance around the globe and this pool of genetic variation serves as the base for selection as well as for plant improvement. Thus, identification, characterization and documentation of the gene pool of medicinal plants are essential for this purpose. Genomic information of many a medicinal plant species has increased rapidly sinc...


Biotechnological production of muconic acid: Current status and future prospects
Muconic acid (MA), a high value-added bio-product with reactive dicarboxylic groups and conjugated double bonds, has garnered increasing interest owing to its potential applications in the manufacture of new functional resins, bio-plastics, food additives, agrochemicals, and pharmaceuticals. At the very least, MA can be used to produce commercially important bulk chemicals such as adipic acid, ...
Efficient expression of a soluble lipid transfer protein (LTP) of Platanus orientalis using short peptide tags, and structural comparison with the natural form
Successful recombinant allergen-based immunotherapy has drawn a great deal of attention to using recombinant allergens for new therapeutic and/or diagnostic strategies. The Escherichia coli (E. coli) expression system is frequently used to produce recombinant allergens; however, protein expression in E. coli often results in inclusion bodies. Here, we focused on the expression of two recombinan...


Treatment of an agrochemical wastewater by combined coagulation and fenton oxidation
Growing environmental concern about the presence of pesticides in water demands their removal, particularly in industrial wastewater effluents. These streams are characterized by high content of organic colloidal matter. Physicochemical treatments based on the combination of coagulation-flocculation processes have been deeply studied. An integral treatment of a non-biodegradable agrochemical wa...
Evaluation of the liquid, solid and total fractions of beer, cider and wine lees as economic nutrient for xylitol production
The final prices of fermentative culture broths must be reduced in order to extend biotechnological processes to an industrial scale. In this way, residual lees from the beer, cider and wine industries were evaluated as a source of nitrogen and micronutrients for xylitol production by Debaryomyces hansenii. The liquid fraction of wine or beer lees was employed as suitable culture media for xyli...

Cantilever arrayed blood pressure sensor for arterial applanation tonometry
The authors developed a cantilever-arrayed blood pressure sensor array fabricated by (111) silicon bulk-micromachining for the non-invasive and continuous measurement of blood pressure. The blood pressure sensor measures the blood pressure based on the change in the resistance of the piezoresistor on a 5-μm-thick-arrayed perforated membrane and 20-μm-thick metal pads. The length a...
Carbon nanotube-based hot-film and temperature sensor assembled by optically-induced dielectrophoresis
The development of carbon nanotube (CNT)-based sensors remains an active area of research. Towards this end, a new method for manipulating CNTs, assembling CNT networks and fabricating CNT-based nanosensors was demonstrated in this study. CNTs were collected and concentrated by optically-induced dielectrophoresis (ODEP) forces and aligned between a pair of electrodes. This assembly was then use...

Effect of molecular structure of sulphonated polyimide membranes on water sorption
Water sorption in sulphonated polyimides with or without ionic block structure was analysed with Feng's new dual mode model. The effect of their molecular structure that determines the chain organisation in the solid materials was analysed by using the model parameters. The model parameters Cp and A' correspond to the sorbed water molecules on the first layer close to the ionic groups and on th...
Specific interactions between functionalised particles and circulating tumour cells
Receptor-ligand binding has been one of the more popular approaches to specifically targeting tumour cells. In this work, targeting efficiency was quantitatively characterized using silica particles functionalized with EpCAM antibodies and EpCAM-expressing BT-20 breast cancer cells. The effects of incubation time and particle concentration on the number of functionalised particles bound to targ...

Use of cell morphology as early bioindicator for viral infection
This study reports a correlation between cellular morphology and the ability of adapting to vesicular stomatitis virus infection. A time-lapse approach was employed to track the individual difference between homologous cells in adapting to viral infection. The authors single-cell analysis indicates that upon viral infection, mature cells that are in spindle shape are less likely to be infected ...
Electrochemical microsensor based on gold nanoparticles modified electrode for total phosphorus determinations in water
This work mainly describes an electrochemical microsensor based on the reduction of molybdophosphate complex for total phosphorus (TP) determinations in water. The microelectrode was fabricated using microelectromechanical systems (MEMS) techniques and porous, branching gold nanoparticles (AuNPs) were electrodeposited on the microelectrode to improve its sensitivity. Calibration of the microsen...
Spatially selective release of aptamer-captured cells by temperature mediation
Isolation of cells from heterogeneous biological samples is critical in both basic biological research and clinical diagnostics. Affinity-based methods, such as those that recognise cells by binding antibodies to cell membrane biomarkers, can be used to achieve specific cell isolation. Microfluidic techniques have been employed to achieve more efficient and effective cell isolation. By employin...
Incorporation of ligand–receptor binding-site models and transistor-based sensors for resolving dissociation constants and number of binding sites
Ligand-receptor binding site model is used to elucidate the binding affinity between ligands and receptors, with transistor-based sensors. AlGaN/GaN high electron mobility transistors (HEMTs) were immobilised with antibodies and human immunodeficiency virus type 1 reverse transcriptase enzymes to detect peptides and human immunodeficiency virus drugs, respectively. The signals generated by the ...