Biology

Androgen receptor DNA methylation regulates the timing and androgen sensitivity of mouse prostate ductal development
Androgen receptor (AR) signaling initiates mouse prostate development by stimulating prostate ductal bud formation and specifying bud patterns. Curiously, however, prostatic bud initiation lags behind the onset of gonadal testosterone synthesis by about three days. This study's objective was to test the hypothesis that DNA methylation controls the timing and scope of prostate ductal development...


Elf5 and Ets2 maintain the mouse extraembryonic ectoderm in a dosage dependent synergistic manner
The ETS superfamily transcription factors Elf5 and Ets2 have both been implicated in the maintenance of the extraembryonic ectoderm (ExE) of the mouse embryo. While homozygous mutants of either gene result in various degrees of ExE tissue loss, heterozygotes are without phenotype. We show here that compound heterozygous mutants exhibit a phenotype intermediate to that of the more severe Elf5-/-...
CbGRiTS: Cerebellar gene regulation in time and space
The mammalian CNS is one of the most complex biological systems to understand at the molecular level. The temporal information from time series transcriptome analysis can serve as a potent source of associative information between developmental processes and regulatory genes. Here, we introduce a new transcriptome database called, Cerebellar Gene Regulation in Time and Space (CbGRiTS). This dat...


Gro/TLE Enables embryonic stem cell differentiation by Repressing pluripotent gene expression
Gro/TLE proteins (TLE1-4) are a family of transcriptional corepressors acting downstream of multiple signalling pathways. Several TLEs are expressed in a dynamic manner throughout embryonic development and at high levels in embryonic stem cells (ESCs). Here we find that Gro/TLE is not required in ESC for sustaining pluripotency and suppressing differentiation genes, but rather is important for ...
Acacieae Benth. (Leguminosae, Mimosoideae) in Minas Gerais, Brazil
Acacieae Benth. (Leguminosae–Mimosoideae) comprises more than 1450 species and is composed of five genera in the current circumscription: Senegalia Raf., Mariosousa Seigler & Ebinger, Vachellia Wight & Arn., Acacia Mill., and Acaciella Britton & Rose. This work describes the diversity of the Tribe Acacieae in the state of Minas Gerais. The studies were based on the analysis of spe...


Lmx1a enhances the therapeutic effect of induced neural stem cells in a Parkinson’s disease mouse model
Lmx1a plays a central role in the specification of dopaminergic (DA) neurons, which potentially could be employed as a key factor for trans-differentiation to DA neurons. In our previous study, we have converted somatic cells directly into neural stem cell – like cells, namely induced neural stem cells (iNSCs), which further can be differentiated into subtypes of neurons and glia in vitro. In...
Degradation of amyloid beta by human induced pluripotent stem cell-derived macrophages expressing Neprilysin-2
The purpose of this study was to evaluate the therapeutic potential of human induced pluripotent stem (iPS) cell-derived macrophage-like cells for Alzheimer's disease (AD). In previous studies, we established the technology to generate macrophage-like myeloid lineage cells with proliferating capacity from human iPS cells, and we designated the cells iPS-ML. iPS-ML reduced the level of Aβ added...

The Notch Delta-4 ligand helps to maintain the quiescence and the short-term reconstitutive potential of haematopoietic progenitor cells through activation of a key gene network
Understanding the role of Notch and its ligands within the different bone marrow niches could shed light on the mechanisms regulating haematopoietic progenitor cells (HPCs) maintenance and self-renewal. Here, we report that murine bone marrow HPCs activation by the vascular Notch Delta-4 ligand maintains a significant proportion of cells specifically in the G0 state. Furthermore, Delta-4/Notch ...
Understanding the complex-I-ty of metformin action: limiting mitochondrial respiration to improve cancer therapy
Metformin has been a first-line treatment for type II diabetes mellitus for decades and is the most widely prescribed antidiabetic drug. Retrospective studies have found that metformin treatment is associated with both reduced cancer diagnoses and cancer-related deaths. Despite the prevalence of metformin use in the clinic, its molecular mechanism of action remains controversial. In a recent is...

A cytogenetic, molecular genetic and morphological study of Patagonian chinchilla mice Euneomys (Rodentia, Cricetidae) in the Southern Central Andes
Euneomys is a genus of sigmodontine rodent endemic to South America, distributed along the Andes of Argentina and Chile and in adjoining Patagonian steppe. Here, we studied specimens of Euneomys from different localities in the Central Andes, Mendoza Province, Argentina. Karyotypic analyses confirm the presence of a new karyomorph of Euneomys chinchilloides, the form 2n = 34, FN = 62–...
Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure
Successful progression through the cell cycle requires spatial and temporal regulation of gene transcript levels and the number, positions and condensation levels of chromosomes. Here we present a high resolution survey of genome interactions in Schizosaccharomyces pombe using synchronized cells to investigate cell cycle dependent changes in genome organization and transcription. Cell cycle dep...

Prevalence of overweight and obesity in older Mexican adults and its association with physical activity and related factors: An analysis of the study on global ageing and adult health
The obesity epidemic in Mexico is increasing and represents a considerable public health challenge. The population aged 50 years and older is also increasing and is not exempt from the obesity rise. We aimed to determine the current prevalence of Body Mass Index (BMI) categories in a sample of Mexicans aged 50 years and older and to test the associations of BMI with physical activity categories...
Variability in Estimated Glomerular Filtration Rate by Area under the Curve Predicts Renal Outcomes in Chronic Kidney Disease
Greater variability in renal function is associated with mortality in patients with chronic kidney disease (CKD). However, few studies have demonstrated the predictive value of renal function variability in relation to renal outcomes. This study investigates the predictive ability of different methods of determining estimated glomerular filtration rate (eGFR) variability for progression to rena...
Differences in Risk Factors for Retinopathy of Prematurity Development in Paired Twins: A Chinese Population Study
To determine the differences in risk factors for retinopathy of prematurity (ROP) in paired twins. Methods. A retrospective medical record review was performed for all paired twins screened for ROP between 2007 and 2012. Screening was offered to very low birth weight (≤1500 grams) and preterm (≤32 weeks) neonates. Twins 1 and 2 were categorized based on the order of delivery. Maternal and n...
Effect of Continuous Cropping Generations on Each Component Biomass of Poplar Seedlings during Different Growth Periods
In order to investigate the change rules and response characteristics of growth status on each component of poplar seedling followed by continuous cropping generations and growth period, we clear the biomass distribution pattern of poplar seedling, adapt continuous cropping, and provide theoretical foundation and technical reference on cultivation management of poplar seedling, the first genera...