Biology

Clinical Cutoffs for Adherence Barriers in Solid Organ Transplant Recipients: How Many Is Too Many?
The current study used multiple statistical methods to determine empirically derived and clinically relevant cutoff scores on the Adolescent Medication Barriers Scale (AMBS) and Parent Medication Barriers Scale (PMBS) to detect adolescents and young adults with solid organ transplants who experienced medication nonadherence or negative medical outcomes. Participants included 71 patients and 80 ...


Relative Mobility of Human Transferrin Domains Accounts for Its Efficient Recognition and Recycling
Human serum transferrin (hTf) transports ferric ions in the blood stream andinflamed mucosal surfaces with high affinity and delivers them to cells viareceptor mediated endocytosis. A typical hTf is folded into two homologouslobes; each lobe is further divided into two similar sized domains. Threedifferent crystal structures of hTf delineate large conformational changesinvolved in iron binding/...
A High Power-Density Mediator-Free Microfluidic Biophotovoltaic Device for Cyanobacterial Cells
Biophotovoltaics has emerged as a promising technology for generatingrenewable energy since it relies on living organisms as inexpensive,self-repairing and readily available catalysts to produce electricity from anabundant resource - sunlight. The efficiency of biophotovoltaic cells, however,has remained significantly lower than that achievable through syntheticmaterials. Here, we devise a plat...


High-throughput screening for modulators of cellular contractile force
When cellular contractile forces are central to pathophysiology, these forcescomprise a logical target of therapy. Nevertheless, existing high-throughputscreens are limited to upstream signaling intermediates with poorly definedrelationship to such a physiological endpoint. Using cellular force as thetarget, here we screened libraries to identify novel drug candidates in thecase of human airway...
The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators
Methylation and de-methylation of histone lysine residues play pivotal roles in mammalian early development; these modifications influence chromatin architecture and regulate gene transcription. Fbxl11 (F-box and leucine-rich repeat 11)/Kdm2a is a histone demethylase that selectively removes mono- and di-methylation from histone H3K36. Previously, two other histone H3K36 demethylases (Jmjd5 or ...


Understanding the shoot apical meristem regulation: A study of the phytohormones, auxin and cytokinin, in rice
Auxin and cytokinin regulate different critical processes involved in plant growth and environmental feedbacks. These plant hormones act either synergistically or antagonistically to control the organisation, formation and maintenance of meristem. Meristem cells can be divided to generate new tissues and organs at the locations of plant postembryonic development. The aboveground plant organs ar...
Sleep disorders, obesity, and aging: the role of orexin
The hypothalamic neuropeptides orexin A and B (hypocretin 1 and 2) are important homeostatic mediators of central control of energy metabolism and maintenance of sleep/wake states. Dysregulation or loss of orexin signaling has been linked to narcolepsy, obesity, and age-related disorders. In this review, we present an overview of our current understanding of orexin function, focusing on sleep d...

Cryoprotective effect of phosphorous-containing phenolic anti-oxidant for the cryopreservation of beluga sperm
A cryoprotective effect of an addition of a new synthetic antioxidant – a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein’s medium. I...
Motility and fertility of cryopreserved semen in Persian sturgeon, Acipenser persicus, stored for 30–60min after thawing
We investigated the effect of storage times of frozen–thawed Persian sturgeon (Acipenser persicus) semen on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30, and 60min at 4°C post-thawing. Frozen thawed semen analyzed immediately after thawing had similar quality characteristics as fresh semen. For cryo...

Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time–temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to determine whether it is vitrified or undergoes phase change transition. When dealing with cryogenic liquids,...
Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system
Adipose-derived stem cells (ADSCs), which are an accessible source of adult stem cells with capacities for self-renewal and differentiation into various cell types, have a promising potential in tissue engineering and regenerative medicine strategies. To meet the clinical demand for ADSCs, cryopreservation has been applied for long-term ADSC preservation. To optimize the addition, removal, free...

Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5′ AMP-activated protein kinase (AMPK)
This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio® as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10mM metformin, 2mM ...
Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation
Clove bud (Syzygium aromaticum) extract was added at concentrations of 0, 35, 75, and 115μg/ml to ovine semen extenders in order to investigate the antioxidant activities of clove bud extract and its effects on semen quality parameters after cryopreservation of ram spermatozoa. The basic extender was composed of Tris, egg yolk, and glycerol. Two other extenders were prepared by substitution of...
Negative inotropic effects of epinephrine in the presence of increased β-adrenoceptor sensitivity during hypothermia in a rat model
Animal studies show reduced inotropic effects of cardiac β-adrenoceptor agonists like epinephrine (Epi) during hypothermia and rewarming, while drugs targeting other pharmacological mechanisms have positive effects. This study therefore aimed to determine β-adrenoceptor sensitivity in isolated cardiomyocytes and investigate hemodynamic effects of Epi and its ability to stimulate cardiac β-ad...
Morphology, cell viability, karyotype, expression of surface markers and plasticity of three human primary cell line cultures before and after the cryostorage in LN2 and GN2
Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the culture characteristics and the loss of viability of three human primary cell line...