Nowadays, there is a tremendous global interest in the biofuels production. However, first generation biofuels have been debated about that energy-crop compete with food crops and thus cause food deficiency and price increases. In this sense, researchers have started looking for potential feedstock for ethanol such as lignocellulosic biomass (e.g., sugar cane bagasse), which does not affect food security. In this paper, the integrated use of sugarcane bagasse is analyzed as raw material for second generation of biofuels production. This case study implements a design and process integration to compare several biorefinery topologies using the typical mass flow rate of.