A quantitative densitometric thin-layer chromatographic method for determination of nefopam hydrochloride in pharmaceutical preparations has been established and validated. Nefopam from the formulations was separated and identified on silica gel 6 F254 TLC plates with chloroform-methanol-glacial acetic acid (9: 2: .1, v/v/v) as mobile phase. Densitometric quantification was performed at absorbance maximum 266 nm. The method was validated for linearity, sensitivity, precision and recovery in accordance with ICH guidelines. The presented method is selective and specific with potential application in pharmaceutical analysis. Nefopam hydrochloride was subjected to acidic and alkaline hydrolysis at different temperatures. As the method could effectively separate the drug from its degradation products, it can be employed as a stability indicating one.
Related Content
Development and Validation of Stability-Indicating Assay Method by UPLC for a Fixed Dose Combination of Atorvastatin and Ezetimibe
A stability-indicating ultra-performance liquid chromatography method was developed and validated for the simultaneous determination of a fixed dose combination of atorvastatin and ezetimibe in bulk drugs. The developed method was successfully applied to the simultaneous quantitative analysis of ...


Quantitative solid phase microextraction – Gas chromatography mass spectrometry analysis of the pesticides lindane, heptachlor and two heptachlor transformation products in groundwater
This paper describes the development and validation of a method for the determination of lindane, heptachlor and two heptachlor transformation products (exo- and endo-heptachlor epoxide) in groundwater. Samples were extracted using a simple solid phase microextraction (SPME) method with a polyacr...
Development and Validation of a Stability Indicating RP-TLC/Densitometric Method for Determination of Loratadine in Bulk and in Tablets
A new rapid, economical and environmental friendly Reversed–Phase Thin-Layer chromatography (RP-TLC)/densitometry has been developed and validated for quantitative determination of Loratadine (LOR) in bulk and in tablets. RP-TLC separation was achieved on aluminium plates precoated with silica ...


The development and validation of a Thin Layer Chromatography densitometry method for the analysis of diclofenac sodium tablets
Diclofenac sodium is a Nonsteroidal Anti-Inflammatory Drug (NSAID) with anti-inflammatory, antipyretic, and analgesic action as a result of the blockade of prostaglandin synthesis by inhibition of the cyclooxygenase enzyme. Analysis of the active pharmaceutical ingredient as well as finished phar...
Development and Validation of a Capillary Zone Electrophoresis Method for the Quantitative Determination of Atypical Antipsychotic Risperidone in Pharmaceutical Dosage forms
A rapid and simple analytical capillary zone electrophoresis (CZE) method has been developed and validated for quantitative determination of atypical antipsychotic drug risperidone in commercially available film-coated tablets. CZE was performed in an uncoated fused-silica capillary 40.2 cm total...

Stability-indicating densitometric determination of some angiotensin II receptor antagonists in presence of their degradation products
A simple, selective, precise, and stability-indicating thin-layer chromatographic method has been developed and validated for analysis of some angiotensin II receptor antagonists (AIIRAs), namely, Losartan potassium (Los-K), Irbesartan (Irb), and Candesartan cilexetil (Cand) in the bulk drug and ...
Development and Validation of a Stability-Indicating RP-HPLC Method for the Simultaneous Estimation of Process Related Impurities and Degradation Products of Rasagiline Mesylate in Pharmaceutical Formulation
A sensitive, stability-indicating gradient reverse phase high-performance liquid chromatography–ultraviolet method has been developed for the quantitative determination of process-related impurities and forced degradation products of rasagiline mesylate in pharmaceutical formulation. Effici...

Development and Validation of a Stability-Indicating RP-HPLC Assay Method and Stress Degradation Studies on Dapiprazole
Dapiprazole (DPZ) was subjected to different stress conditions prescribed by the International Conference on Harmonization. A stability-indicating high-performance liquid chromatography method was developed for the analysis of the drug in the presence of its degradation products. The degradation ...
Development and validation of a quantitative LC-tandem MS assay for hexadeca-4,7,10,13-tetraenoic acid in human and mouse plasma
Upon exposure to platinum analogs, mesenchymal stem cells were recently found to excrete minute amounts of specific lipid mediators that induce chemotherapy resistance. One of these lipids is hexadeca-4,7,10,13-tetraenoic acid (FA(16:4)n-3). Importantly, FA(16:4)n-3 is present in high concentrati...

Development of a novel pyrolysis-gas chromatography/mass spectrometry method for the analysis of poly(lactic acid) thermal degradation products
Thermal degradation of PLA is a complex process since it comprises many simultaneous reactions. The use of analytical techniques, such as differential scanning calorimetry (DSC) and thermogravimetry (TGA), yields useful information but a more sensitive analytical technique would be necessary to i...