A method is proposed for the quantification of hydrazine by reversed-phase chromatography after its derivatization with naphthalene-2,3-dialdehyde. The conditions of derivatization and the chromatography separation on a Zorbax Eclipse XDB-C8 column in the gradient mode are optimized. The derivatization and chromatography analysis take 1 and 16 min, respectively. If fluorimetry detection (λex = 273 nm, λem = 5 nm) is used and the injection volume is 1 μL, the detection limit is .5 μg/L. The procedure is applicable to the quantification of hydrazine in natural waters and soil extracts. A simple and rapid procedure is elaborated for the determination of .1–5 μg/L hydrazine in natural waters, RSD = 12% (n = 3).
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