Here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of Nantskaya and Perfektzya variety with the highest level of recombinant human interferon-2b accumulation exhibiting the highest level of plant protein extract antiviral activity (up to 12.8 × 103 IU/mg TSP). The antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from the parent organisms. However, the antiviral activity level of suspension culture extracts (up to 4.42 × 103 IU/mg TSP) and Ri-root ones (up to 4.42 × 103 IU/mg TSP) appeared to be comparable to analogical data of antiviral activity of transgenic carrot leaf extracts, this way the described cultures could be possibly used for comparatively speedy obtaining of recombinant therapeutic protein for curing and preventing of virus diseases.
In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven ...
Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesized by photosynthetic organisms. Of the four forms ([alpha], beta, [gamma] and [delta]), [alpha]-tocopherol is the major vitamin E form present in green plan...
This review addresses methods of obtaining secondary metabolites from plant cell suspension and hairy root cultures and their exudates, particularly the physiological mechanisms of secondary metabolites release and trafficking. The efficiency for product recovery of metabolites can be increased b...
Interest in Taxus species has increased since paclitaxel, an anticancer drug, was isolated from the bark of Taxus brevifolia (Pacific yew) in the 1960's. Great effort has been carried out to establish an efficient callus cultures of Taxus species. Culture media must be optimized for each Taxus sp...
Treatment of common and debilitating degenerative cartilage diseases particularly osteoarthritis is a clinical challenge because of the limited capacity of the tissue for self-repair. Because of their unlimited capacity for self-renewal and ability to differentiate into multiple lineages, human e...
Human mast cells are tissue resident cells with a principal role in allergic disorders. Cross-linking of the high-affinity receptor for immunoglobulin E (FcRI) results in release of inflammatory mediators initiating the clinical symptoms of allergy and anaphylaxis. Much of our knowledge regarding...
The cell adhesion extracellular matrix (ECM), and the cell adhesive system mediate various adhesion molecules. Cell adhesion regulates proliferation and differentiation. However, the cell adhesive systems between titanium and epithelial cells are still not clearly understood. In this study, we cu...
Data presented herein provides a rapid and efficient method for Agrobacterium rhizogenes-mediated genetic transformation of Arnebia hispidissima for hairy root cultures as well as for enhancing Shikonin production. Etiolated explants viz. shoot tip, nodal, leaf and internodal segments were co-cul...
submitted by trthelokkamor 1 year and 3 months ago
The NIMA-related kinases (Neks) are widespread among eukaryotes. In mammalians they represent an evolutionarily conserved family of 11 serine/threonine kinases, with 40-45% amino acid sequence identity to the Aspergillus nidulans mitotic regulator NIMA within their catalytic domains. Neks have ce...
Primary astrocyte cell cultures have become a valuable tool for studies of signaling pathways that regulate astrocyte physiology, reactivity and function. However, differences in culture preparation affect data reproducibility. The aim of this work was to define optimal conditions for obtaining p...
