Recently released sequence information on Chinese Hamster Ovary (CHO) cells promises to not only facilitate our understanding of these industrially important cell factories through direct analysis of the sequence, but also to enhance existing methodologies and allow new tools to be developed. In this paper we demonstrate the utilisation of CHO specific sequence information to improve mass spectrometry (MS) based proteomic identification. The use of various CHO specific databases enabled the identification of 282 additional proteins, thus increasing the total number of identified proteins by 40-50%, depending on the sample source and methods used. In addition, a considerable portion of those proteins that were identified previously based on inter-species sequence homology were now identified by a larger number of peptides matched, thus increasing the confidence of identification. The new sequence information offers improved interpretation of proteomic analyses and will, in the years to come, prove vital to unravelling the CHO proteome. Biotechnol. Bioeng.
We produced human growth hormone (hGH), a protein that stimulates growth and cell reproduction, in genetically engineered soybean [Glycine max (L.) Merrill] seeds. Utilising the alpha prime (α′) subunit of β-conglycinin tissue-specific promoter from soybean and the α-Coixin signal peptide fr...
The electrical energy requirements of the Maltese Islands are met in their entirety by two oil-fired electrical generation plants. In view of this complete dependence upon fossil fuels, investigations into the prospects of diversifying electrical generation by resorting to renewable energy techno...
Uplink power control in 3GPP UTRAN long term evolution (LTE) networks consists of a closed-loop scheme around an open-loop point of operation. The uplink performance of the network is decisively influenced by power control. This paper provides insight into the uplink power control procedure and i...
A two-dimensional gel electrophoresis (2-DE) based proteomic approach has been used to study the Medicago truncatula-Uromyces striatus interaction. The 2-DE leaf protein profile of three M. truncatula genotypes displaying different phenotypes (susceptible and showing prehaustorial and posthaustor...
Continuous manufacturing systems used within the steel industry involve different machines and processes that are arranged in a sequence of operations in order to manufacture the products. The steel industry is generally a capital-intensive industry and, because of high capital investment, the ut...
Models of fuel cell based combined heat and power systems, used in building energy performance simulation codes, are often based on simple black or grey box models. To model a specific device, input data from experiments are often required for calibration. This paper presents an approach for the ...
Chloromethane gas is produced naturally in the phyllosphere, the environmental compartment defined as the above ground parts of vegetation, which hosts a rich bacterial flora. Chloromethane may serve as a growth substrate for specialised aerobic methylotrophic bacteria, which have been isolated f...
The rising burden of obesity in Tonga is alarming. The promotion of healthy behaviours and environments requires immediate urgent action and a multi-sectoral approach. A 3 year community based study titled the Ma'alahi Youth Project (MYP) conducted in Tonga from 2005-2008 aimed to increase the ca...
The flourishing number of publications on the use of isotope ratio mass spectrometry (IRMS) in forensic science denotes the enthusiasm and the attraction generated by this technology. IRMS has demonstrated its potential to distinguish chemically identical compounds coming from different sources. ...
Pay-for-performance systems raise concerns regarding inequity in health care because providers might select patients for whom targets can easily be reached. This paper aims to describe the evolution of pre-existing (in)equity in health care in the period after the introduction of the Quality and ...
