The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (PET) and nylon that had been grafted with PNIPAAm were tested for their ability to support the proliferation and subsequent thermal release of HC04 and HepG2 hepatocytes. Hepatocyte viability and proliferation was estimated using the Alamar Blue assay and Hoechst 33258 total DNA quantification. The assays revealed that the pure and grafted non-woven scaffolds maintained the hepatocytes within the matrix and promoted 3D proliferation comparable to that of the commercially available AlgimatrixTM alginate scaffold. Albumin production and selected cytochrome P450 genes expression was found to be superior in cells growing on pure and grafted non-woven PP scaffolds as compared to cells grown as a 2D monolayer. Two scaffolds, namely, PP-g-PNIPAAm-A and PP-g-PNIPAAm-B were identified as having far superior thermal release capabilities; releasing the majority of the cells from the matrices within 2 h. This is the first report for the development of 3D non-woven, thermo-responsive scaffolds able to release cells from the matrix without the use of any enzymatic assistance or scaffold degradation. Biotechnol. Bioeng.
We describe here the advantages of oligo(ethylene glycol)-based (co)polymers for preparing thermoresponsive materials as diverse as polymer-enzyme bio-hybrids, injectable hydrogels, capsules for drug-release, modified magnetic particles for in vivo utilization, cell-culture substrates, antibacter...
The combination of thermo-sensitive polymer and natural macromolecules such as collagen type I can produce biomimetic scaffolds with enhanced properties for clinical translation. This study describes the preparation of semi-Interpenetrated Networks (semi-IPNs) of crosslinked poly(N-isopropylacryl...
A novel thermo-responsive and biocompatible copolymer hydrogel based on N-isopropylacrylamide(NIPAAm) and galactosylated acrylate(GAC) were synthesized successfully by the free radical copolymerization. The copolymer hydrogel poly(NIPAAm-co-GAC) exhibits reversible temperature-response at ∼3...
Cell culture substrates of defined topography have emerged as powerful tools with which to investigate cell mechanobiology, but current technologies only allow passive control of substrate properties. Here we present a thermo-responsive cell culture system that uses shape memory polymer (SMP) sub...
Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell–cell and cell–matrix interactions. Intact c...
Poly(N-isopropylacrylamide) (PNIPAAm)-grafted poly(dimethylsiloxane) (PDMS) offers an inexpensive, biocompatible, oxygen permeable, and easily microtextured thermo-responsive substrate for producing cell sheets. This study introduces a method of grafting PNIPAAm onto microtextured PDMS that is su...
Myocardial infarction (MI) results in the death of cardiomyocytes (CM) followed by scar formation and pathological remodeling of the heart. We propose that chitosan conjugated with the angiopoietin-1 derived peptide, QHREDGS, and mixed with collagen I forms a thermo-responsive hydrogel better sui...
The aim of this work is to explore the ability of poly (N-isopropylacrylamide) (NIPAAm) with galactosylated acrylate (GAC) hydrogels as cell scaffolds for HL-7702 cells to proliferate, keep function of albumin and urea synthesis and detach. The results indicate poly (NIPAAm-co-GAC) hydrogels with...
A series of temperature-sensitive poly(CSA-co-NIPAAm) membranes that were suitable for cell culture and confluent cell sheets detachment were prepared. The membranes with thermo-responsive surface properties were synthesized by the copolymerization of acrylic acid-derivatized chitosan (CSA) and N...
The unique physical property of thermo-responsive polymer (TRP) has recently prompted its increasing applications in tissue engineering. On the other hand, TRP has not been exploited for potential applications in quantitative cell screening against external stimulations. In this study, TRP is app...
