Fourier Transform Infrared (FT-IR) spectroscopic imaging is a promising method that enables the analysis of spatial distribution of biochemical components within histological sections. However, analysis of FT-IR spectroscopic data is complicated since absorption peaks often overlap with each other. Second derivative spectroscopy is a technique which enhances the separation of overlapping peaks. The objective of this study was to evaluate the specificity of the second derivative peaks for the main tissue components of articular cartilage (AC),i.e.collagen and proteoglycans (PGs).Materials and methodsHistological bovine AC sections were measured before and after enzymatic removal of PGs. Both formalin-fixed sections (n=10) and cryosections (n=6) were investigated. Relative changes in the second derivative peak heights caused by the removal of PGs were calculated for both sample groups. The results showed that numerous peaks,e.g., peaks located at 1202 cmand 1336 cm, altered less than 5% in the experiment. These peaks were assumed to be specific for collagen. In contrast, two peaks located at 1064 cmand 1376 cmwere seen to alter notably, approximately 50% or more. These peaks were regarded to be specific for PGs. The changes were greater in cryosections than formalin-fixed sections.ConclusionsThe results of this study suggest that the second derivative spectroscopy offers a practical and more specific method than routinely used absorption spectrum analysis methods to obtain compositional information on AC with FT-IR spectroscopic imaging.
Due to their natural origin, biocompatibility, and non-toxicity, cellulose nanocrystals are promising candidates for applications in nanomedicine. Highly fluorescent nanocellulosic material was prepared via surface modification of cellulose nanocrystals with 2,2′:6′,2″-terpyridine side chai...
Hypocrellin A (HA) is a naturally occurring perylenequinone pigment with bright red colour. The inclusion complex of HA with hydroxypropyl-I2-cyclodextrin was prepared successfully by lyophilization method for the first time. Phase solubility studies indicated that the inclusion complex was forme...
Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) microspectroscopy was applied for detection of colon cancer according to the spectral features of colon tissues. Supervised classification models can be trained to identify the tissue type based on the spectroscopic fingerprint. A...
Waxy corn starch was esterified with 2-octen-1-ylsuccinic anhydride (OSA) using response surface methodology. The molecular structure and paste properties were also investigated. Results indicated that the optimum parameters for esterification were as follows: reaction period 4 h, temperature 36....
Since trans fat labeling requirements became mandatory in the US and many other countries, there has been a need for rapid and accurate analytical methodologies that can facilitate compliance with the various regulations. The determination of total trans fatty acids by mid-infrared ...
Vibrational spectroscopy has a long history as an important spectroscopic method in chemical and pharmaceutical analysis. Instrumentation for infrared (IR) spectroscopy was revolutionized by the introduction of Fourier Transform Infrared (FTIR) spectrometers. In addition, easier sampling combined...
We describe the application of a recently discovered family of materials called quantum clusters, which are sub-nanometer particles composed of a few atoms with well-defined molecular formulae, exhibiting intense absorption and emission in the visible region in metal ion sensing, taking Ag25as an...
Various contrast agents (Magnevist®, Gadovist® and Multihance®) loaded into polymeric nanocapsules were synthesized by the inverse miniemulsion technique. The relaxivity of the resultant contrast agents was assessed at 1.5 T magnetic field strength. The ionic relaxivity of the contrast a...
The focus of this paper is the application of Fourier transform infrared spectroscopy (FTIR) and near infrared-tuneable diode laser spectroscopy (NIR-TDLAS) as in-situ tools to monitor the formation of gas phase products during atomic layer deposition (ALD). Two studies are chosen to highlight th...
A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate and lipid contents of microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by a...
