submitted by gaodolapi 1 year and 8 months ago - Topic: Chemical Engineering
High level expression of a recombinant gene results in growth arrest, followed by overgrowth by non-productive derivatives. Two methods are described for the isolation of E. coli BL21* strains that are improved hosts for recombinant protein production. Both are based upon the observations (i) tha...
submitted by KamronGo 1 year and 7 months ago - Topic: Chemical Engineering
Agarose gels with immobilized amine-based ligands L-lysine (Lys) and poly-L-lysine (PLL) exhibited the ability to adsorb human serum proteins and to separate immunoglobulin G (IgG) in nonretained chromatographic fractions (negative chromatography). The effect of the buffer system and pH on IgG pu...
submitted by majtobo 1 year and 7 months ago - Topic: Chemical Engineering
Metabolic profiling of new drugs is limited by the difficulty in obtaining sufficient quantities of minor metabolites for definitive structural identification. Biocatalytic methods offer the potential to produce metabolites that are difficult to synthesize by traditional medicinal chemistry. We h...
submitted by merno 1 year and 7 months ago - Topic: Chemical Engineering
Recombinant protein production significantly improved in the past three decades. Novel expression systems were developed, growth conditions optimised and the technology and thus monitoring and analysis significantly enhanced. However, the studies of bacterial cell disruption were more or less neg...
submitted by DomiJud 1 year and 6 months ago - Topic: Chemical Engineering
A variety of fluorescent nanoparticles have been developed for demanding applications such as optical biosensing and fluorescence imaging in live cells. Silica-based fluorescent nanoparticles offer diverse advantages for biological applications. For example, they can be used as labeling probes du...
submitted by dege2 1 year and 4 months ago - Topic: Chemical Engineering
A chimeric cyanophycin synthetase gene composed of thecphATecoding region from the cyanobacteriumThermosynechococcus elongatusBP-1, the constitutive 35S promoter and the plastid targeting sequence of the integral photosystem II protein PsbY was transferred to the tobacco variety Petit Havanna SRI...
submitted by NoahAle 7 months ago - Topic: Chemical Engineering
The aim of this study was to select autochthonous microorganisms that are able to biosorb heavy metals in aqueous systems. Forty-eight strains, included fungi, yeasts and bacteria, were isolated from water and sludge samples from urban and industrial wastewater treatment plants in the province of...
submitted by RossChris 4 months ago - Topic: Chemical Engineering
This review covers key technologies being applied to stem cell separation, and also highlights exciting new approaches in this field. First, we will cover conventional separation methods that are commercially available and have been widely adapted. These methods include fluorescence-activated cel...
submitted by agagag 2 months ago - Topic: Chemical Engineering
With the strong dissolution ability to biomaterials, ionic liquids can lead to a better release of the active and valuable ingredients embedded in plant matrices. Accordingly, an integrated microwave-assisted ionic liquids treatment followed by hydro-distillation (MILT-MHD) for isolating the esse...
submitted by RoberZach 1 month ago - Topic: Chemical Engineering
In the Netherlands there are around 400 “Seveso” sites that fall under the Dutch Major Hazards Decree (BRZO) 1999. Between 2006 and 2010 the Dutch Labour Inspectorate’s Directorate for Major Hazard Control completed investigations of 118 loss of containment incidents involving hazardous sub...