Biology

New Insights into the FLPergic Complements of Parasitic Nematodes: informing deorphanisation approaches
FMRFamide-like peptide (FLP) receptors are appealing as putative anthelmintic targets. To date, 31 flp-encoding genes have been identified in Caenorhabditis elegans and thirteen FLP-activated G-protein coupled receptors (FLP-GPCRs) have been reported. The lack of knowledge on FLPs and FLP-GPCRs in parasites impedes their functional characterisation and chemotherapeutic exploitation. Using homol...


The membrane complexome of a new Pseudomonas strain during growth on lysogeny broth medium and medium containing glucose or phenol
In this study, we have performed a systematic analysis of Pseudomonas sp. strain phDV1 membrane protein complexes by growing the strain in lysogeny broth medium, and medium containing glucose or phenol as sole carbon sources. In order to study the membrane complexome, we developed an approach for the extraction and the analysis of the membrane protein complexes in native conditions. Our strateg...
Two approaches for proving linearizability of multiset
Linearizability is a key correctness criterion for concurrent software. In our previous work, we have introduced local proof obligations, which, by showing a refinement between an abstract specification and its implementation, imply linearizability of the implementation. The refinement is shown via a process local simulation. We have incorporated the approach of verifying linearizability based ...


A sensitive amperometric detection of dopamine agonist drug pramipexole at functionalized multi-walled carbon nanotubes (f-MWCNTs) modified electrode
The electrochemical detection of dopaminergic agonist drug pramipexole dihydrochloride monohydrate (PPX) has been investigated by cyclic voltammetric (CV) and amperometric i–t techniques at functionalized multi-walled carbon nanotubes-modified glassy carbon electrode. For the first time, a sensitive and rapid electrochemical method was developed for the determination of PPX. The surface morph...
Improving the electrochemistry and microstructure of nickel electrode by deposition on anodized titanium substrate for the electrocatalytic oxidation of methanol and ethanol
The electrodeposition process of nickel and the substrate used for the electrodeposition can be improved to obtain an effective catalyst for methanol oxidation. Thus, nanoparticles of nickel have been uniformly electrodeposited on the surface of previously anodized titanium at 5 V during 1 h. The optimized microstructure has been studied by using scanning electron microscopy (SEM), X-ray phot...


The effect of different concentrations of Na2SnO3 on the electrochemical behaviors of the Mg-8Li electrode
In this research, the effect of the different concentrations of NaSnO3 as the electrolyte additive in 0.7 mol L−1 NaCl solution on the electrochemical performances of the magnesium-8lithium (Mg-8Li) electrode are investigated by methods of potentiodynamic polarization, potentiostatic current-time, electrochemical impedance technique, and scanning electron microscopy (SEM). The corrosion res...
The Drosophila chmp1 protein determines wing cell fate through regulation of Epidermal Growth Factor Receptor signaling
Receptor down-regulation by the multivesicular body (MVB) pathway is critical for many cellular signaling events. MVB generation is mediated by the highly conserved ESCRT (0, I, II, and III) protein complexes. Chmp1 is an ESCRT-III component and a putative tumor suppressor in humans. However, published data on Chmp1 activity are conflicting and its role during tissue development is not well def...

The role of heparan sulfate proteoglycans in optic disc and stalk morphogenesis
Heparan sulfate proteoglycans (HSPG) are important for embryonic development via the regulation of gradient formation and signaling of multiple growth factors and morphogens. Previous studies have shown that Bmp/Shh/Fgf signaling are required for the regionalization of the optic vesicle (OV) and for the closure of the optic fissure (OF), the disturbance of which underlie ocular anomalies such a...
First proposed panels on acute leukemia for four-color immunophenotyping by flow cytometry from the brazilian group of flow cytometry-GBCFLUX
Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with a wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories are equipped with four-color flow cytometer facilities. Based on this fact, the Brazilian Group of Flow Cytometry (Grupo Brasileir...

Thiol-reactivity of the fungicide maneb
Maneb (MB) is a manganese-containing ethylene bis-dithiocarbamate fungicide that is implicated as an environmental risk factor for Parkinson’s disease, especially in combination with paraquat (PQ). Dithiocarbamates inhibit aldehyde dehydrogenases, but the relationship of this to the combined toxicity of MB + PQ is unclear because PQ is an oxidant and MB activates Nrf2 and increases cellular...
Oxygen delivery, consumption, and conversion to reactive oxygen species in experimental models of diabetic retinopathy
Retinal tissue receives its supply of oxygen from two sources – the retinal and choroidal circulations. Decreases in retinal blood flow occur in the early stages of diabetes, with the eventual development of hypoxia thought to contribute to pathological neovascularization. Oxygen consumption in the retina has been found to decrease in diabetes, possibly due to either a reduction in neuronal m...

Redox status in mammalian cells and stem cells during culture in vitro: Critical roles of Nrf2 and cystine transporter activity in maintenance of redox balance
Culturing cells and tissues in vitro has provided valuable insights into the molecular mechanisms regulating redox signaling in cells with implications for medicine. However, standard culture techniques maintain mammalian cells in vitro under an artificial physicochemical environment such as ambient air and 5% CO2. Oxidative stress is caused by the rapid oxidation of cysteine to cystine in cult...
The complex role of inflammasomes in the pathogenesis of Inflammatory Bowel Diseases–lessons learned from experimental models
Inflammasomes are a large family of multiprotein complexes recognizing pathogen-associated molecular pattern molecules (PAMPs) and damage-associated molecular patterns (DAMPs). This leads to caspase-1 activation, promoting the secretion of mature IL-1β, IL-18 and under certain conditions even induce pyroptosis. Inflammatory Bowel Diseases (IBD) is associated with alterations in microbiota comp...
The cytoplasmic side of p53’s oncosuppressive activities
The tumor suppressor p53 is a transcription factor that in response to a plethora of stress stimuli activates a complex and context-dependent cellular response ultimately protecting genome integrity. In the last two decades, the discovery of cytoplasmic p53 localization has driven an intense research on its extra-nuclear functions. The ability to induce apoptosis acting directly at mitochondria...
The changes induced in the physiological, biochemical and anatomical characteristics of Vicia faba by the exogenous application of proline under seawater stress
The depletion of fresh water resources leads to the utilisation of various alternative sources of water, such as seawater. In this regard, the foliar application of proline is one of the alternative shotgun approaches to increase plant stress tolerance. A pot experiment was conducted in the wire house of the National Research Centre, Dokki, Cairo, Egypt, during the winter season of 2010–201Th...