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A Novel Adjustment Method for Shearer Traction Speed through Integration of T-S Cloud Inference Network and Improved PSO
In order to efficiently and accurately adjust the shearer traction speed, a novel approach based on Takagi-Sugeno (T-S) cloud inference network (CIN) and improved particle swarm optimization (IPSO) is proposed. The T-S CIN is built through the combination of cloud model and T-S fuzzy neural network. Moreover, the IPSO algorithm employs parameter automation adjustment strategy and velocity reset...


BrainCheck ¿ a very brief tool to detect incipient cognitive decline: optimized case-finding combining patient- and informant-based data
Optimal identification of subtle cognitive impairment in the primary care setting requires a very brief tool combining (a) patients’ subjective impairments, (b) cognitive testing, and (c) information from informants. The present study developed a new, very quick and easily administered case-finding tool combining these assessments (‘BrainCheck’) and tested the feasibility and validity of ...
Sleep disorders, obesity, and aging: the role of orexin
The hypothalamic neuropeptides orexin A and B (hypocretin 1 and 2) are important homeostatic mediators of central control of energy metabolism and maintenance of sleep/wake states. Dysregulation or loss of orexin signaling has been linked to narcolepsy, obesity, and age-related disorders. In this review, we present an overview of our current understanding of orexin function, focusing on sleep d...


Cryoprotective effect of phosphorous-containing phenolic anti-oxidant for the cryopreservation of beluga sperm
A cryoprotective effect of an addition of a new synthetic antioxidant – a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein’s medium. I...
Motility and fertility of cryopreserved semen in Persian sturgeon, Acipenser persicus, stored for 30–60min after thawing
We investigated the effect of storage times of frozen–thawed Persian sturgeon (Acipenser persicus) semen on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30, and 60min at 4°C post-thawing. Frozen thawed semen analyzed immediately after thawing had similar quality characteristics as fresh semen. For cryo...


Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time–temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to determine whether it is vitrified or undergoes phase change transition. When dealing with cryogenic liquids,...
Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system
Adipose-derived stem cells (ADSCs), which are an accessible source of adult stem cells with capacities for self-renewal and differentiation into various cell types, have a promising potential in tissue engineering and regenerative medicine strategies. To meet the clinical demand for ADSCs, cryopreservation has been applied for long-term ADSC preservation. To optimize the addition, removal, free...

Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5′ AMP-activated protein kinase (AMPK)
This study evaluated the effect of the use of hypometabolic TRIS extenders in the presence or the absence of AMPK activators as well as the utilization of high cooling rates in the refrigeration step on the freezability of stallion sperm. Twelve ejaculates were cryopreserved using Botucrio® as a control extender and a basic TRIS extender (HM-0) separately supplemented with 10mM metformin, 2mM ...
Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation
Clove bud (Syzygium aromaticum) extract was added at concentrations of 0, 35, 75, and 115μg/ml to ovine semen extenders in order to investigate the antioxidant activities of clove bud extract and its effects on semen quality parameters after cryopreservation of ram spermatozoa. The basic extender was composed of Tris, egg yolk, and glycerol. Two other extenders were prepared by substitution of...

Negative inotropic effects of epinephrine in the presence of increased β-adrenoceptor sensitivity during hypothermia in a rat model
Animal studies show reduced inotropic effects of cardiac β-adrenoceptor agonists like epinephrine (Epi) during hypothermia and rewarming, while drugs targeting other pharmacological mechanisms have positive effects. This study therefore aimed to determine β-adrenoceptor sensitivity in isolated cardiomyocytes and investigate hemodynamic effects of Epi and its ability to stimulate cardiac β-ad...
Morphology, cell viability, karyotype, expression of surface markers and plasticity of three human primary cell line cultures before and after the cryostorage in LN2 and GN2
Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the culture characteristics and the loss of viability of three human primary cell line...

Cryotolerance of Day 2 or Day 6 in vitro produced ovine embryos after vitrification by Cryotop or Spatula methods
This study was conducted to evaluate the cryotolerance of in vitro produced ovine embryos submitted to vitrification at different developmental stages using two methods of minimum volume and rapid cooling rate. Embryos were vitrified at an early stage (2 to 8-cells) on Day 2 or at an advanced stage (morulae and blastocysts) on Day 6 after in vitro fertilization. Vitrification procedure consiste...
Reverse Engineering Point Clouds to Fit Tensor Product B-Spline Surfaces by Blending Local Fits
Being able to reverse engineer from point cloud data to obtain 3D models isimportant in modeling. As our main contribution, we present a new method toobtain a tensor product B-spline representation from point cloud data byfitting surfaces to appropriately segmented data. By blending multiple localfits our method is more efficient than existing techniques, with the ability todeal with more detai...
Algebraic reputation model RepRank and its application to spambot detection
Due to popularity surge social networks became lucrative targets for spammersand guerilla marketers, who are trying to game ranking systems and broadcasttheir messages at little to none cost. Ranking systems, for example Twitter'sTrends, can be gamed by scripted users also called bots, who are automaticallyor semi-automatically twitting essentially the same message. Judging by theprices and abu...
Clustering evolving data using kernel-based methods
In this thesis, we propose several modelling strategies to tackle evolvingdata in different contexts. In the framework of static clustering, we start byintroducing a soft kernel spectral clustering (SKSC) algorithm, which canbetter deal with overlapping clusters with respect to kernel spectralclustering (KSC) and provides more interpretable outcomes. Afterwards, a wholestrategy based upon KSC f...